Loading…
Expression of an extracellular ribonuclease gene increases resistance to Cucumber mosaic virus in tobacco
The apoplast plays an important role in plant defense against pathogens. Some extracellular PR-4 proteins possess ribonuclease activity and may directly inhibit the growth of pathogenic fungi. It is likely that extracellular RNases can also protect plants against some viruses with RNA genomes. Howev...
Saved in:
Published in: | BMC plant biology 2016-11, Vol.16 (Suppl 3), p.246-246, Article 246 |
---|---|
Main Authors: | , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
cited_by | cdi_FETCH-LOGICAL-c605t-9950fd63e5b6d41f4fedbf4c5ae8b5797d503690c4801211dc3d291b32c811763 |
---|---|
cites | cdi_FETCH-LOGICAL-c605t-9950fd63e5b6d41f4fedbf4c5ae8b5797d503690c4801211dc3d291b32c811763 |
container_end_page | 246 |
container_issue | Suppl 3 |
container_start_page | 246 |
container_title | BMC plant biology |
container_volume | 16 |
creator | Sugawara, Teppei Trifonova, Ekaterina A Kochetov, Alex V Kanayama, Yoshinori |
description | The apoplast plays an important role in plant defense against pathogens. Some extracellular PR-4 proteins possess ribonuclease activity and may directly inhibit the growth of pathogenic fungi. It is likely that extracellular RNases can also protect plants against some viruses with RNA genomes. However, many plant RNases are multifunctional and the direct link between their ribonucleolytic activity and antiviral defense still needs to be clarified. In this study, we evaluated the resistance of Nicotiana tabacum plants expressing a non-plant single-strand-specific extracellular RNase against Cucumber mosaic virus.
Severe mosaic symptoms and shrinkage were observed in the control non-transgenic plants 10 days after inoculation with Cucumber mosaic virus (CMV), whereas such disease symptoms were suppressed in the transgenic plants expressing the RNase gene. In a Western blot analysis, viral proliferation was observed in the uninoculated upper leaves of control plants, whereas virus levels were very low in those of transgenic plants. These results suggest that resistance against CMV was increased by the expression of the heterologous RNase gene.
We have previously shown that tobacco plants expressing heterologous RNases are characterized by high resistance to Tobacco mosaic virus. In this study, we demonstrated that elevated levels of extracellular RNase activity resulted in increased resistance to a virus with a different genome organization and life cycle. Thus, we conclude that the pathogen-induced expression of plant apoplastic RNases may increase non-specific resistance against viruses with RNA genomes. |
doi_str_mv | 10.1186/s12870-016-0928-8 |
format | article |
fullrecord | <record><control><sourceid>gale_doaj_</sourceid><recordid>TN_cdi_doaj_primary_oai_doaj_org_article_fb172b181c454f30a79ddac0c6c433cf</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A470585323</galeid><doaj_id>oai_doaj_org_article_fb172b181c454f30a79ddac0c6c433cf</doaj_id><sourcerecordid>A470585323</sourcerecordid><originalsourceid>FETCH-LOGICAL-c605t-9950fd63e5b6d41f4fedbf4c5ae8b5797d503690c4801211dc3d291b32c811763</originalsourceid><addsrcrecordid>eNptkstu1DAUhiMEoqXwAGyQJTawSPHxLfayGhUYqRISl7Xl-DLyKIkHO6nK2-MwpTAIe2H7-PuPdY7_pnkJ-BJAincFiOxwi0G0WBHZykfNObAOWkKIevzX_qx5VsoeY-gkU0-bMyIBc8XVeROv7w7ZlxLThFJAZkL-bs7G-mFYBpNRjn2aFjt4Uzza-cmjONm8ngqqulhmM1mP5oQ2i13G3mc0pmKiRbcxL6XS9a431qbnzZNghuJf3K8Xzbf31183H9ubTx-2m6ub1grM51YpjoMT1PNeOAaBBe_6wCw3Xva8U53jmAqFLZMYCICz1BEFPSVWAnSCXjTbY16XzF4fchxN_qGTifpXIOWdNnmOtSQdeuhIDxIs4yxQbDrlnLHYCssotaHmenPMdcjp--LLrMdY1t6Yyael6PoJwKWoo6Kv_0H3aclTrbRSjFMQAuM_1M7U9-MU0trsNam-Yh3mklNCK3X5H6pO58do0-RDrPETwdsTQWXm-o87s5Sit18-n7JwZG1OpWQfHnoEWK-20kdb6WorvdpKy6p5dV_c0o_ePSh--4j-BO8fxcY</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1845316600</pqid></control><display><type>article</type><title>Expression of an extracellular ribonuclease gene increases resistance to Cucumber mosaic virus in tobacco</title><source>Publicly Available Content Database</source><source>PubMed Central</source><creator>Sugawara, Teppei ; Trifonova, Ekaterina A ; Kochetov, Alex V ; Kanayama, Yoshinori</creator><creatorcontrib>Sugawara, Teppei ; Trifonova, Ekaterina A ; Kochetov, Alex V ; Kanayama, Yoshinori</creatorcontrib><description>The apoplast plays an important role in plant defense against pathogens. Some extracellular PR-4 proteins possess ribonuclease activity and may directly inhibit the growth of pathogenic fungi. It is likely that extracellular RNases can also protect plants against some viruses with RNA genomes. However, many plant RNases are multifunctional and the direct link between their ribonucleolytic activity and antiviral defense still needs to be clarified. In this study, we evaluated the resistance of Nicotiana tabacum plants expressing a non-plant single-strand-specific extracellular RNase against Cucumber mosaic virus.
Severe mosaic symptoms and shrinkage were observed in the control non-transgenic plants 10 days after inoculation with Cucumber mosaic virus (CMV), whereas such disease symptoms were suppressed in the transgenic plants expressing the RNase gene. In a Western blot analysis, viral proliferation was observed in the uninoculated upper leaves of control plants, whereas virus levels were very low in those of transgenic plants. These results suggest that resistance against CMV was increased by the expression of the heterologous RNase gene.
We have previously shown that tobacco plants expressing heterologous RNases are characterized by high resistance to Tobacco mosaic virus. In this study, we demonstrated that elevated levels of extracellular RNase activity resulted in increased resistance to a virus with a different genome organization and life cycle. Thus, we conclude that the pathogen-induced expression of plant apoplastic RNases may increase non-specific resistance against viruses with RNA genomes.</description><identifier>ISSN: 1471-2229</identifier><identifier>EISSN: 1471-2229</identifier><identifier>DOI: 10.1186/s12870-016-0928-8</identifier><identifier>PMID: 28105959</identifier><language>eng</language><publisher>England: BioMed Central Ltd</publisher><subject>Analysis ; Animals ; Apoplast ; Bacteria, Phytopathogenic ; Cucumovirus - physiology ; Disease Resistance - genetics ; Gene Expression Regulation, Plant ; Genes ; Nicotiana - genetics ; Nicotiana - virology ; Plant Immunity - genetics ; Plants ; Plants, Genetically Modified - genetics ; Plants, Genetically Modified - virology ; Prevention ; Resistance ; Resistance (Psychoanalysis) ; Ribonuclease ; Ribonuclease, Pancreatic - genetics ; Ribonuclease, Pancreatic - metabolism ; RNA viruses ; RNases ; Tobacco (Plant)</subject><ispartof>BMC plant biology, 2016-11, Vol.16 (Suppl 3), p.246-246, Article 246</ispartof><rights>COPYRIGHT 2016 BioMed Central Ltd.</rights><rights>Copyright BioMed Central 2016</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c605t-9950fd63e5b6d41f4fedbf4c5ae8b5797d503690c4801211dc3d291b32c811763</citedby><cites>FETCH-LOGICAL-c605t-9950fd63e5b6d41f4fedbf4c5ae8b5797d503690c4801211dc3d291b32c811763</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.proquest.com/docview/1845316600?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>314,780,784,25753,27924,27925,37012,37013,44590</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28105959$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sugawara, Teppei</creatorcontrib><creatorcontrib>Trifonova, Ekaterina A</creatorcontrib><creatorcontrib>Kochetov, Alex V</creatorcontrib><creatorcontrib>Kanayama, Yoshinori</creatorcontrib><title>Expression of an extracellular ribonuclease gene increases resistance to Cucumber mosaic virus in tobacco</title><title>BMC plant biology</title><addtitle>BMC Plant Biol</addtitle><description>The apoplast plays an important role in plant defense against pathogens. Some extracellular PR-4 proteins possess ribonuclease activity and may directly inhibit the growth of pathogenic fungi. It is likely that extracellular RNases can also protect plants against some viruses with RNA genomes. However, many plant RNases are multifunctional and the direct link between their ribonucleolytic activity and antiviral defense still needs to be clarified. In this study, we evaluated the resistance of Nicotiana tabacum plants expressing a non-plant single-strand-specific extracellular RNase against Cucumber mosaic virus.
Severe mosaic symptoms and shrinkage were observed in the control non-transgenic plants 10 days after inoculation with Cucumber mosaic virus (CMV), whereas such disease symptoms were suppressed in the transgenic plants expressing the RNase gene. In a Western blot analysis, viral proliferation was observed in the uninoculated upper leaves of control plants, whereas virus levels were very low in those of transgenic plants. These results suggest that resistance against CMV was increased by the expression of the heterologous RNase gene.
We have previously shown that tobacco plants expressing heterologous RNases are characterized by high resistance to Tobacco mosaic virus. In this study, we demonstrated that elevated levels of extracellular RNase activity resulted in increased resistance to a virus with a different genome organization and life cycle. Thus, we conclude that the pathogen-induced expression of plant apoplastic RNases may increase non-specific resistance against viruses with RNA genomes.</description><subject>Analysis</subject><subject>Animals</subject><subject>Apoplast</subject><subject>Bacteria, Phytopathogenic</subject><subject>Cucumovirus - physiology</subject><subject>Disease Resistance - genetics</subject><subject>Gene Expression Regulation, Plant</subject><subject>Genes</subject><subject>Nicotiana - genetics</subject><subject>Nicotiana - virology</subject><subject>Plant Immunity - genetics</subject><subject>Plants</subject><subject>Plants, Genetically Modified - genetics</subject><subject>Plants, Genetically Modified - virology</subject><subject>Prevention</subject><subject>Resistance</subject><subject>Resistance (Psychoanalysis)</subject><subject>Ribonuclease</subject><subject>Ribonuclease, Pancreatic - genetics</subject><subject>Ribonuclease, Pancreatic - metabolism</subject><subject>RNA viruses</subject><subject>RNases</subject><subject>Tobacco (Plant)</subject><issn>1471-2229</issn><issn>1471-2229</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>PIMPY</sourceid><sourceid>DOA</sourceid><recordid>eNptkstu1DAUhiMEoqXwAGyQJTawSPHxLfayGhUYqRISl7Xl-DLyKIkHO6nK2-MwpTAIe2H7-PuPdY7_pnkJ-BJAincFiOxwi0G0WBHZykfNObAOWkKIevzX_qx5VsoeY-gkU0-bMyIBc8XVeROv7w7ZlxLThFJAZkL-bs7G-mFYBpNRjn2aFjt4Uzza-cmjONm8ngqqulhmM1mP5oQ2i13G3mc0pmKiRbcxL6XS9a431qbnzZNghuJf3K8Xzbf31183H9ubTx-2m6ub1grM51YpjoMT1PNeOAaBBe_6wCw3Xva8U53jmAqFLZMYCICz1BEFPSVWAnSCXjTbY16XzF4fchxN_qGTifpXIOWdNnmOtSQdeuhIDxIs4yxQbDrlnLHYCssotaHmenPMdcjp--LLrMdY1t6Yyael6PoJwKWoo6Kv_0H3aclTrbRSjFMQAuM_1M7U9-MU0trsNam-Yh3mklNCK3X5H6pO58do0-RDrPETwdsTQWXm-o87s5Sit18-n7JwZG1OpWQfHnoEWK-20kdb6WorvdpKy6p5dV_c0o_ePSh--4j-BO8fxcY</recordid><startdate>20161116</startdate><enddate>20161116</enddate><creator>Sugawara, Teppei</creator><creator>Trifonova, Ekaterina A</creator><creator>Kochetov, Alex V</creator><creator>Kanayama, Yoshinori</creator><general>BioMed Central Ltd</general><general>BioMed Central</general><general>BMC</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>ISR</scope><scope>3V.</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>DOA</scope></search><sort><creationdate>20161116</creationdate><title>Expression of an extracellular ribonuclease gene increases resistance to Cucumber mosaic virus in tobacco</title><author>Sugawara, Teppei ; Trifonova, Ekaterina A ; Kochetov, Alex V ; Kanayama, Yoshinori</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c605t-9950fd63e5b6d41f4fedbf4c5ae8b5797d503690c4801211dc3d291b32c811763</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Analysis</topic><topic>Animals</topic><topic>Apoplast</topic><topic>Bacteria, Phytopathogenic</topic><topic>Cucumovirus - physiology</topic><topic>Disease Resistance - genetics</topic><topic>Gene Expression Regulation, Plant</topic><topic>Genes</topic><topic>Nicotiana - genetics</topic><topic>Nicotiana - virology</topic><topic>Plant Immunity - genetics</topic><topic>Plants</topic><topic>Plants, Genetically Modified - genetics</topic><topic>Plants, Genetically Modified - virology</topic><topic>Prevention</topic><topic>Resistance</topic><topic>Resistance (Psychoanalysis)</topic><topic>Ribonuclease</topic><topic>Ribonuclease, Pancreatic - genetics</topic><topic>Ribonuclease, Pancreatic - metabolism</topic><topic>RNA viruses</topic><topic>RNases</topic><topic>Tobacco (Plant)</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sugawara, Teppei</creatorcontrib><creatorcontrib>Trifonova, Ekaterina A</creatorcontrib><creatorcontrib>Kochetov, Alex V</creatorcontrib><creatorcontrib>Kanayama, Yoshinori</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biological Sciences</collection><collection>Agriculture Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>PML(ProQuest Medical Library)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>Directory of Open Access Journals</collection><jtitle>BMC plant biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sugawara, Teppei</au><au>Trifonova, Ekaterina A</au><au>Kochetov, Alex V</au><au>Kanayama, Yoshinori</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression of an extracellular ribonuclease gene increases resistance to Cucumber mosaic virus in tobacco</atitle><jtitle>BMC plant biology</jtitle><addtitle>BMC Plant Biol</addtitle><date>2016-11-16</date><risdate>2016</risdate><volume>16</volume><issue>Suppl 3</issue><spage>246</spage><epage>246</epage><pages>246-246</pages><artnum>246</artnum><issn>1471-2229</issn><eissn>1471-2229</eissn><abstract>The apoplast plays an important role in plant defense against pathogens. Some extracellular PR-4 proteins possess ribonuclease activity and may directly inhibit the growth of pathogenic fungi. It is likely that extracellular RNases can also protect plants against some viruses with RNA genomes. However, many plant RNases are multifunctional and the direct link between their ribonucleolytic activity and antiviral defense still needs to be clarified. In this study, we evaluated the resistance of Nicotiana tabacum plants expressing a non-plant single-strand-specific extracellular RNase against Cucumber mosaic virus.
Severe mosaic symptoms and shrinkage were observed in the control non-transgenic plants 10 days after inoculation with Cucumber mosaic virus (CMV), whereas such disease symptoms were suppressed in the transgenic plants expressing the RNase gene. In a Western blot analysis, viral proliferation was observed in the uninoculated upper leaves of control plants, whereas virus levels were very low in those of transgenic plants. These results suggest that resistance against CMV was increased by the expression of the heterologous RNase gene.
We have previously shown that tobacco plants expressing heterologous RNases are characterized by high resistance to Tobacco mosaic virus. In this study, we demonstrated that elevated levels of extracellular RNase activity resulted in increased resistance to a virus with a different genome organization and life cycle. Thus, we conclude that the pathogen-induced expression of plant apoplastic RNases may increase non-specific resistance against viruses with RNA genomes.</abstract><cop>England</cop><pub>BioMed Central Ltd</pub><pmid>28105959</pmid><doi>10.1186/s12870-016-0928-8</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
fulltext | fulltext |
identifier | ISSN: 1471-2229 |
ispartof | BMC plant biology, 2016-11, Vol.16 (Suppl 3), p.246-246, Article 246 |
issn | 1471-2229 1471-2229 |
language | eng |
recordid | cdi_doaj_primary_oai_doaj_org_article_fb172b181c454f30a79ddac0c6c433cf |
source | Publicly Available Content Database; PubMed Central |
subjects | Analysis Animals Apoplast Bacteria, Phytopathogenic Cucumovirus - physiology Disease Resistance - genetics Gene Expression Regulation, Plant Genes Nicotiana - genetics Nicotiana - virology Plant Immunity - genetics Plants Plants, Genetically Modified - genetics Plants, Genetically Modified - virology Prevention Resistance Resistance (Psychoanalysis) Ribonuclease Ribonuclease, Pancreatic - genetics Ribonuclease, Pancreatic - metabolism RNA viruses RNases Tobacco (Plant) |
title | Expression of an extracellular ribonuclease gene increases resistance to Cucumber mosaic virus in tobacco |
url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-23T13%3A32%3A30IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_doaj_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Expression%20of%20an%20extracellular%20ribonuclease%20gene%20increases%20resistance%20to%20Cucumber%20mosaic%20virus%20in%20tobacco&rft.jtitle=BMC%20plant%20biology&rft.au=Sugawara,%20Teppei&rft.date=2016-11-16&rft.volume=16&rft.issue=Suppl%203&rft.spage=246&rft.epage=246&rft.pages=246-246&rft.artnum=246&rft.issn=1471-2229&rft.eissn=1471-2229&rft_id=info:doi/10.1186/s12870-016-0928-8&rft_dat=%3Cgale_doaj_%3EA470585323%3C/gale_doaj_%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c605t-9950fd63e5b6d41f4fedbf4c5ae8b5797d503690c4801211dc3d291b32c811763%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=1845316600&rft_id=info:pmid/28105959&rft_galeid=A470585323&rfr_iscdi=true |