Loading…

Biocomputational Assessment of Natural Compounds as a Potent Inhibitor to Quorum Sensors in Ralstonia solanacearum

is among the most damaging bacterial phytopathogens with a wide number of hosts and a broad geographic distribution worldwide. The pathway of phenotype conversion (Phc) is operated by quorum-sensing signals and modulated through the (R)-methyl 3-hydroxypalmitate (3-OH PAME) in . However, the molecul...

Full description

Saved in:
Bibliographic Details
Published in:Molecules (Basel, Switzerland) Switzerland), 2022-05, Vol.27 (9), p.3034
Main Authors: Kumar, Sunil, Ahmad, Khurshid, Behera, Santosh Kumar, Nagrale, Dipak T, Chaurasia, Anurag, Yadav, Manoj Kumar, Murmu, Sneha, Jha, Yachana, Rajawat, Mahendra Vikram Singh, Malviya, Deepti, Singh, Udai B, Shankar, Raja, Tripathy, Minaketan, Singh, Harsh Vardhan
Format: Article
Language:English
Subjects:
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c493t-d81d9c6ff6426240a9c7bd39f10a71cdb65f1654c9550f83b54f8ab04b979e9c3
cites cdi_FETCH-LOGICAL-c493t-d81d9c6ff6426240a9c7bd39f10a71cdb65f1654c9550f83b54f8ab04b979e9c3
container_end_page
container_issue 9
container_start_page 3034
container_title Molecules (Basel, Switzerland)
container_volume 27
creator Kumar, Sunil
Ahmad, Khurshid
Behera, Santosh Kumar
Nagrale, Dipak T
Chaurasia, Anurag
Yadav, Manoj Kumar
Murmu, Sneha
Jha, Yachana
Rajawat, Mahendra Vikram Singh
Malviya, Deepti
Singh, Udai B
Shankar, Raja
Tripathy, Minaketan
Singh, Harsh Vardhan
description is among the most damaging bacterial phytopathogens with a wide number of hosts and a broad geographic distribution worldwide. The pathway of phenotype conversion (Phc) is operated by quorum-sensing signals and modulated through the (R)-methyl 3-hydroxypalmitate (3-OH PAME) in . However, the molecular structures of the Phc pathway components are not yet established, and the structural consequences of 3-OH PAME on quorum sensing are not well studied. In this study, 3D structures of quorum-sensing proteins of the Phc pathway (PhcA and PhcR) were computationally modeled, followed by the virtual screening of the natural compounds library against the predicted active site residues of PhcA and PhcR proteins that could be employed in limiting signaling through 3-OH PAME. Two of the best scoring common ligands ZINC000014762512 and ZINC000011865192 for PhcA and PhcR were further analyzed utilizing orbital energies such as HOMO and LUMO, followed by molecular dynamics simulations of the complexes for 100 ns to determine the ligands binding stability. The findings indicate that ZINC000014762512 and ZINC000011865192 may be capable of inhibiting both PhcA and PhcR. We believe that, after further validation, these compounds may have the potential to disrupt bacterial quorum sensing and thus control this devastating phytopathogenic bacterial pathogen.
doi_str_mv 10.3390/molecules27093034
format article
fullrecord <record><control><sourceid>proquest_doaj_</sourceid><recordid>TN_cdi_doaj_primary_oai_doaj_org_article_fb183688b797497bb40ae39e1689ce92</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><doaj_id>oai_doaj_org_article_fb183688b797497bb40ae39e1689ce92</doaj_id><sourcerecordid>2663049879</sourcerecordid><originalsourceid>FETCH-LOGICAL-c493t-d81d9c6ff6426240a9c7bd39f10a71cdb65f1654c9550f83b54f8ab04b979e9c3</originalsourceid><addsrcrecordid>eNplkltvFCEUxydGY2v1A_hiSHzxZRUGhoEXk7qxuknj3WcCDLRsZjgrFxO_fdlubVpNSCDn_M7_cC5d95zg15RK_GaB2dk6u9yPWFJM2YPumLAeryhm8uGd91H3JOctxj1hZHjcHdFh4JwKetyldwEsLLtadAkQ9YxOc3Y5Ly4WBB590qWmZl03BmqcMtLtoC9Q9sAmXgYTCiRUAH2tkOqCvruYIWUUIvqm51wgBo0yzDpq63QjnnaPfHO4Zzf3Sffz7P2P9cfV-ecPm_Xp-coySctqEmSSlnvPWc97hrW0o5mo9ATrkdjJ8METPjArhwF7Qc3AvNAGMyNH6aSlJ93moDuB3qpdCotOfxTooK4NkC6UTiXY2SlviKBcCDPKkcnRmJbOUekIF9I62TettwetXTWLm2wrvnXlnuh9TwyX6gJ-K0lwz_le4NWNQIJf1eWilpCtm1tbHNSsGsQEJrzHDX35D7qFmtporqn9OMUoG0UOlE2Qc3L-9jMEq_12qP-2o8W8uFvFbcTfdaBXgoa6Dg</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2663049879</pqid></control><display><type>article</type><title>Biocomputational Assessment of Natural Compounds as a Potent Inhibitor to Quorum Sensors in Ralstonia solanacearum</title><source>Open Access: PubMed Central</source><source>Publicly Available Content (ProQuest)</source><creator>Kumar, Sunil ; Ahmad, Khurshid ; Behera, Santosh Kumar ; Nagrale, Dipak T ; Chaurasia, Anurag ; Yadav, Manoj Kumar ; Murmu, Sneha ; Jha, Yachana ; Rajawat, Mahendra Vikram Singh ; Malviya, Deepti ; Singh, Udai B ; Shankar, Raja ; Tripathy, Minaketan ; Singh, Harsh Vardhan</creator><creatorcontrib>Kumar, Sunil ; Ahmad, Khurshid ; Behera, Santosh Kumar ; Nagrale, Dipak T ; Chaurasia, Anurag ; Yadav, Manoj Kumar ; Murmu, Sneha ; Jha, Yachana ; Rajawat, Mahendra Vikram Singh ; Malviya, Deepti ; Singh, Udai B ; Shankar, Raja ; Tripathy, Minaketan ; Singh, Harsh Vardhan</creatorcontrib><description>is among the most damaging bacterial phytopathogens with a wide number of hosts and a broad geographic distribution worldwide. The pathway of phenotype conversion (Phc) is operated by quorum-sensing signals and modulated through the (R)-methyl 3-hydroxypalmitate (3-OH PAME) in . However, the molecular structures of the Phc pathway components are not yet established, and the structural consequences of 3-OH PAME on quorum sensing are not well studied. In this study, 3D structures of quorum-sensing proteins of the Phc pathway (PhcA and PhcR) were computationally modeled, followed by the virtual screening of the natural compounds library against the predicted active site residues of PhcA and PhcR proteins that could be employed in limiting signaling through 3-OH PAME. Two of the best scoring common ligands ZINC000014762512 and ZINC000011865192 for PhcA and PhcR were further analyzed utilizing orbital energies such as HOMO and LUMO, followed by molecular dynamics simulations of the complexes for 100 ns to determine the ligands binding stability. The findings indicate that ZINC000014762512 and ZINC000011865192 may be capable of inhibiting both PhcA and PhcR. We believe that, after further validation, these compounds may have the potential to disrupt bacterial quorum sensing and thus control this devastating phytopathogenic bacterial pathogen.</description><identifier>ISSN: 1420-3049</identifier><identifier>EISSN: 1420-3049</identifier><identifier>DOI: 10.3390/molecules27093034</identifier><identifier>PMID: 35566383</identifier><language>eng</language><publisher>Switzerland: MDPI AG</publisher><subject>Bacteria ; Bacterial Proteins - metabolism ; Binding sites ; Energy ; Gene expression ; Gene Expression Regulation, Bacterial ; Geographical distribution ; Ligands ; Metabolites ; molecular docking ; Molecular dynamics ; Molecular orbitals ; Molecular structure ; natural compounds ; Pathogens ; PhcA and PhcR ; Phenotypes ; Proteins ; Quorum sensing ; Quorum Sensing - genetics ; Ralstonia solanacearum ; Simulation ; Virulence</subject><ispartof>Molecules (Basel, Switzerland), 2022-05, Vol.27 (9), p.3034</ispartof><rights>2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2022 by the authors. 2022</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c493t-d81d9c6ff6426240a9c7bd39f10a71cdb65f1654c9550f83b54f8ab04b979e9c3</citedby><cites>FETCH-LOGICAL-c493t-d81d9c6ff6426240a9c7bd39f10a71cdb65f1654c9550f83b54f8ab04b979e9c3</cites><orcidid>0000-0002-1095-8445 ; 0000-0002-3834-6686 ; 0000-0003-3219-0171</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/2663049879/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/2663049879?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,25753,27924,27925,37012,37013,44590,53791,53793,75126</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/35566383$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kumar, Sunil</creatorcontrib><creatorcontrib>Ahmad, Khurshid</creatorcontrib><creatorcontrib>Behera, Santosh Kumar</creatorcontrib><creatorcontrib>Nagrale, Dipak T</creatorcontrib><creatorcontrib>Chaurasia, Anurag</creatorcontrib><creatorcontrib>Yadav, Manoj Kumar</creatorcontrib><creatorcontrib>Murmu, Sneha</creatorcontrib><creatorcontrib>Jha, Yachana</creatorcontrib><creatorcontrib>Rajawat, Mahendra Vikram Singh</creatorcontrib><creatorcontrib>Malviya, Deepti</creatorcontrib><creatorcontrib>Singh, Udai B</creatorcontrib><creatorcontrib>Shankar, Raja</creatorcontrib><creatorcontrib>Tripathy, Minaketan</creatorcontrib><creatorcontrib>Singh, Harsh Vardhan</creatorcontrib><title>Biocomputational Assessment of Natural Compounds as a Potent Inhibitor to Quorum Sensors in Ralstonia solanacearum</title><title>Molecules (Basel, Switzerland)</title><addtitle>Molecules</addtitle><description>is among the most damaging bacterial phytopathogens with a wide number of hosts and a broad geographic distribution worldwide. The pathway of phenotype conversion (Phc) is operated by quorum-sensing signals and modulated through the (R)-methyl 3-hydroxypalmitate (3-OH PAME) in . However, the molecular structures of the Phc pathway components are not yet established, and the structural consequences of 3-OH PAME on quorum sensing are not well studied. In this study, 3D structures of quorum-sensing proteins of the Phc pathway (PhcA and PhcR) were computationally modeled, followed by the virtual screening of the natural compounds library against the predicted active site residues of PhcA and PhcR proteins that could be employed in limiting signaling through 3-OH PAME. Two of the best scoring common ligands ZINC000014762512 and ZINC000011865192 for PhcA and PhcR were further analyzed utilizing orbital energies such as HOMO and LUMO, followed by molecular dynamics simulations of the complexes for 100 ns to determine the ligands binding stability. The findings indicate that ZINC000014762512 and ZINC000011865192 may be capable of inhibiting both PhcA and PhcR. We believe that, after further validation, these compounds may have the potential to disrupt bacterial quorum sensing and thus control this devastating phytopathogenic bacterial pathogen.</description><subject>Bacteria</subject><subject>Bacterial Proteins - metabolism</subject><subject>Binding sites</subject><subject>Energy</subject><subject>Gene expression</subject><subject>Gene Expression Regulation, Bacterial</subject><subject>Geographical distribution</subject><subject>Ligands</subject><subject>Metabolites</subject><subject>molecular docking</subject><subject>Molecular dynamics</subject><subject>Molecular orbitals</subject><subject>Molecular structure</subject><subject>natural compounds</subject><subject>Pathogens</subject><subject>PhcA and PhcR</subject><subject>Phenotypes</subject><subject>Proteins</subject><subject>Quorum sensing</subject><subject>Quorum Sensing - genetics</subject><subject>Ralstonia solanacearum</subject><subject>Simulation</subject><subject>Virulence</subject><issn>1420-3049</issn><issn>1420-3049</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>PIMPY</sourceid><sourceid>DOA</sourceid><recordid>eNplkltvFCEUxydGY2v1A_hiSHzxZRUGhoEXk7qxuknj3WcCDLRsZjgrFxO_fdlubVpNSCDn_M7_cC5d95zg15RK_GaB2dk6u9yPWFJM2YPumLAeryhm8uGd91H3JOctxj1hZHjcHdFh4JwKetyldwEsLLtadAkQ9YxOc3Y5Ly4WBB590qWmZl03BmqcMtLtoC9Q9sAmXgYTCiRUAH2tkOqCvruYIWUUIvqm51wgBo0yzDpq63QjnnaPfHO4Zzf3Sffz7P2P9cfV-ecPm_Xp-coySctqEmSSlnvPWc97hrW0o5mo9ATrkdjJ8METPjArhwF7Qc3AvNAGMyNH6aSlJ93moDuB3qpdCotOfxTooK4NkC6UTiXY2SlviKBcCDPKkcnRmJbOUekIF9I62TettwetXTWLm2wrvnXlnuh9TwyX6gJ-K0lwz_le4NWNQIJf1eWilpCtm1tbHNSsGsQEJrzHDX35D7qFmtporqn9OMUoG0UOlE2Qc3L-9jMEq_12qP-2o8W8uFvFbcTfdaBXgoa6Dg</recordid><startdate>20220509</startdate><enddate>20220509</enddate><creator>Kumar, Sunil</creator><creator>Ahmad, Khurshid</creator><creator>Behera, Santosh Kumar</creator><creator>Nagrale, Dipak T</creator><creator>Chaurasia, Anurag</creator><creator>Yadav, Manoj Kumar</creator><creator>Murmu, Sneha</creator><creator>Jha, Yachana</creator><creator>Rajawat, Mahendra Vikram Singh</creator><creator>Malviya, Deepti</creator><creator>Singh, Udai B</creator><creator>Shankar, Raja</creator><creator>Tripathy, Minaketan</creator><creator>Singh, Harsh Vardhan</creator><general>MDPI AG</general><general>MDPI</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0002-1095-8445</orcidid><orcidid>https://orcid.org/0000-0002-3834-6686</orcidid><orcidid>https://orcid.org/0000-0003-3219-0171</orcidid></search><sort><creationdate>20220509</creationdate><title>Biocomputational Assessment of Natural Compounds as a Potent Inhibitor to Quorum Sensors in Ralstonia solanacearum</title><author>Kumar, Sunil ; Ahmad, Khurshid ; Behera, Santosh Kumar ; Nagrale, Dipak T ; Chaurasia, Anurag ; Yadav, Manoj Kumar ; Murmu, Sneha ; Jha, Yachana ; Rajawat, Mahendra Vikram Singh ; Malviya, Deepti ; Singh, Udai B ; Shankar, Raja ; Tripathy, Minaketan ; Singh, Harsh Vardhan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c493t-d81d9c6ff6426240a9c7bd39f10a71cdb65f1654c9550f83b54f8ab04b979e9c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Bacteria</topic><topic>Bacterial Proteins - metabolism</topic><topic>Binding sites</topic><topic>Energy</topic><topic>Gene expression</topic><topic>Gene Expression Regulation, Bacterial</topic><topic>Geographical distribution</topic><topic>Ligands</topic><topic>Metabolites</topic><topic>molecular docking</topic><topic>Molecular dynamics</topic><topic>Molecular orbitals</topic><topic>Molecular structure</topic><topic>natural compounds</topic><topic>Pathogens</topic><topic>PhcA and PhcR</topic><topic>Phenotypes</topic><topic>Proteins</topic><topic>Quorum sensing</topic><topic>Quorum Sensing - genetics</topic><topic>Ralstonia solanacearum</topic><topic>Simulation</topic><topic>Virulence</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kumar, Sunil</creatorcontrib><creatorcontrib>Ahmad, Khurshid</creatorcontrib><creatorcontrib>Behera, Santosh Kumar</creatorcontrib><creatorcontrib>Nagrale, Dipak T</creatorcontrib><creatorcontrib>Chaurasia, Anurag</creatorcontrib><creatorcontrib>Yadav, Manoj Kumar</creatorcontrib><creatorcontrib>Murmu, Sneha</creatorcontrib><creatorcontrib>Jha, Yachana</creatorcontrib><creatorcontrib>Rajawat, Mahendra Vikram Singh</creatorcontrib><creatorcontrib>Malviya, Deepti</creatorcontrib><creatorcontrib>Singh, Udai B</creatorcontrib><creatorcontrib>Shankar, Raja</creatorcontrib><creatorcontrib>Tripathy, Minaketan</creatorcontrib><creatorcontrib>Singh, Harsh Vardhan</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health &amp; Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>AUTh Library subscriptions: ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health &amp; Medical Complete (Alumni)</collection><collection>Health &amp; Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Publicly Available Content (ProQuest)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>Directory of Open Access Journals</collection><jtitle>Molecules (Basel, Switzerland)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kumar, Sunil</au><au>Ahmad, Khurshid</au><au>Behera, Santosh Kumar</au><au>Nagrale, Dipak T</au><au>Chaurasia, Anurag</au><au>Yadav, Manoj Kumar</au><au>Murmu, Sneha</au><au>Jha, Yachana</au><au>Rajawat, Mahendra Vikram Singh</au><au>Malviya, Deepti</au><au>Singh, Udai B</au><au>Shankar, Raja</au><au>Tripathy, Minaketan</au><au>Singh, Harsh Vardhan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Biocomputational Assessment of Natural Compounds as a Potent Inhibitor to Quorum Sensors in Ralstonia solanacearum</atitle><jtitle>Molecules (Basel, Switzerland)</jtitle><addtitle>Molecules</addtitle><date>2022-05-09</date><risdate>2022</risdate><volume>27</volume><issue>9</issue><spage>3034</spage><pages>3034-</pages><issn>1420-3049</issn><eissn>1420-3049</eissn><abstract>is among the most damaging bacterial phytopathogens with a wide number of hosts and a broad geographic distribution worldwide. The pathway of phenotype conversion (Phc) is operated by quorum-sensing signals and modulated through the (R)-methyl 3-hydroxypalmitate (3-OH PAME) in . However, the molecular structures of the Phc pathway components are not yet established, and the structural consequences of 3-OH PAME on quorum sensing are not well studied. In this study, 3D structures of quorum-sensing proteins of the Phc pathway (PhcA and PhcR) were computationally modeled, followed by the virtual screening of the natural compounds library against the predicted active site residues of PhcA and PhcR proteins that could be employed in limiting signaling through 3-OH PAME. Two of the best scoring common ligands ZINC000014762512 and ZINC000011865192 for PhcA and PhcR were further analyzed utilizing orbital energies such as HOMO and LUMO, followed by molecular dynamics simulations of the complexes for 100 ns to determine the ligands binding stability. The findings indicate that ZINC000014762512 and ZINC000011865192 may be capable of inhibiting both PhcA and PhcR. We believe that, after further validation, these compounds may have the potential to disrupt bacterial quorum sensing and thus control this devastating phytopathogenic bacterial pathogen.</abstract><cop>Switzerland</cop><pub>MDPI AG</pub><pmid>35566383</pmid><doi>10.3390/molecules27093034</doi><orcidid>https://orcid.org/0000-0002-1095-8445</orcidid><orcidid>https://orcid.org/0000-0002-3834-6686</orcidid><orcidid>https://orcid.org/0000-0003-3219-0171</orcidid><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 1420-3049
ispartof Molecules (Basel, Switzerland), 2022-05, Vol.27 (9), p.3034
issn 1420-3049
1420-3049
language eng
recordid cdi_doaj_primary_oai_doaj_org_article_fb183688b797497bb40ae39e1689ce92
source Open Access: PubMed Central; Publicly Available Content (ProQuest)
subjects Bacteria
Bacterial Proteins - metabolism
Binding sites
Energy
Gene expression
Gene Expression Regulation, Bacterial
Geographical distribution
Ligands
Metabolites
molecular docking
Molecular dynamics
Molecular orbitals
Molecular structure
natural compounds
Pathogens
PhcA and PhcR
Phenotypes
Proteins
Quorum sensing
Quorum Sensing - genetics
Ralstonia solanacearum
Simulation
Virulence
title Biocomputational Assessment of Natural Compounds as a Potent Inhibitor to Quorum Sensors in Ralstonia solanacearum
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-23T00%3A39%3A13IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_doaj_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Biocomputational%20Assessment%20of%20Natural%20Compounds%20as%20a%20Potent%20Inhibitor%20to%20Quorum%20Sensors%20in%20Ralstonia%20solanacearum&rft.jtitle=Molecules%20(Basel,%20Switzerland)&rft.au=Kumar,%20Sunil&rft.date=2022-05-09&rft.volume=27&rft.issue=9&rft.spage=3034&rft.pages=3034-&rft.issn=1420-3049&rft.eissn=1420-3049&rft_id=info:doi/10.3390/molecules27093034&rft_dat=%3Cproquest_doaj_%3E2663049879%3C/proquest_doaj_%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c493t-d81d9c6ff6426240a9c7bd39f10a71cdb65f1654c9550f83b54f8ab04b979e9c3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=2663049879&rft_id=info:pmid/35566383&rfr_iscdi=true