Antioxidant Guided Fractionation of Blackberry Polyphenols Show Synergistic Role of Catechins and Ellagitannins

In the present study, blackberry extract was prepared using a previously optimized solid-liquid extraction method in 70% aqueous acetone aimed at the recovery of its principal phenolics. Subsequently, 0.5 g of freeze-dried extract was subjected to flash chromatography fractionation, which was conduc...

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Bibliographic Details
Published in:Molecules (Basel, Switzerland) Switzerland), 2023-02, Vol.28 (4), p.1933
Main Authors: Tzima, Katerina, Putsakum, Gontorn, Rai, Dilip K
Format: Article
Language:English
Subjects:
Anthocyanin
Anthocyanins
Anthocyanins - chemistry
antioxidant indices
Antioxidants
Antioxidants - chemistry
Automation
Berries
Blackberries
Catechin
Catechin - analysis
Chromatography
Chromatography, High Pressure Liquid
Chromatography, Liquid
ellagitannins
flash chromatography fractionation
Food
Fractionation
Fruit - chemistry
Fruits
Hydrolyzable Tannins - analysis
Instrument industry
LC-MS/MS
Mass spectrometry
Phenolic compounds
phenolics
Phenols
Phenols - chemistry
Plant Extracts - chemistry
Polyphenols
Polyphenols - analysis
Rubus
Scientific imaging
Tandem Mass Spectrometry
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Summary:In the present study, blackberry extract was prepared using a previously optimized solid-liquid extraction method in 70% aqueous acetone aimed at the recovery of its principal phenolics. Subsequently, 0.5 g of freeze-dried extract was subjected to flash chromatography fractionation, which was conducted on a C18 column using a binary solvent system of water and methanol at 10 mL/min. The total phenolic content (TPC), 2,2-diphenyl-1-picrylhydrazyl (DPPH), and ferric reducing antioxidant power (FRAP) activities of the obtained 42 flash fractions were determined, and a strong positive correlation ( ≥ 0.986) was exhibited among them. Furthermore, the graph of the antioxidant indices of the flash fractions resembled the flash chromatogram, suggesting a good correlation among the compounds within the chromatographic peaks and the antioxidant indices. LC-MS/MS identified as many 28 phenolics, including cinnamtannin A2 reported for the first time in blackberries. This study further established the role of dominant anthocyanins (cyanidin-3- -glucoside and cyanidin-3- -rutinoside), but uniquely those of ellagitannins and catechins on the antioxidant capacity of blackberries.
ISSN:1420-3049
1420-3049
DOI:10.3390/molecules28041933