Identification of ethnomedicinally important Kaempferia L. (Zingiberaceae) species based on morphological traits and suitable DNA region

Nuclear ribosomal internal transcribed spacer (ITS) sequences and morphological characteristics were used to identify medicinally important Kaempferia species cultivated in Peninsular Malaysia. Six species were evaluated for parameters such as nucleotide diversity (0.458) and estimated values of tra...

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Bibliographic Details
Published in:Current plant biology 2018-09, Vol.14, p.50-55
Main Authors: Labrooy, Catherine Dharshini, Abdullah, Thohirah Lee, Stanslas, Johnson
Format: Article
Language:English
Subjects:
Dendrogram
DNA barcode
ITS4
ITS5
Maximum parsimony
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Summary:Nuclear ribosomal internal transcribed spacer (ITS) sequences and morphological characteristics were used to identify medicinally important Kaempferia species cultivated in Peninsular Malaysia. Six species were evaluated for parameters such as nucleotide diversity (0.458) and estimated values of transition/transversion bias (0.89) using sequence data. Maximum parsimony (MP) analysis inferred divergence pattern in the Kaempferia genus. The resulting phylogenetic tree was compared to one formed using morphological traits. Morphological and molecular data both show 3 distinctive groupings within selected Kaempferia species. ITS 4 and 5 sequences are proposed as DNA barcodes for identification of Kaempferia species. In terms of morphological traits, plant habit, rhizome colour and leaf variation can be used for preliminary identification of this genus.
ISSN:2214-6628
2214-6628
DOI:10.1016/j.cpb.2018.09.004