Inhibitory effects of Colocasia esculenta (L.) Schott constituents on aldose reductase

The goal of this study was to determine the rat lens aldose reductase-inhibitory effects of 95% ethanol extracts from the leaves of C. esculenta and, its organic solvent soluble fractions, including the dichloromethane (CH2Cl2), ethyl acetate (EtOAc), n-butanol (BuOH) and water (H2O) layers, using d...

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Published in:Molecules (Basel, Switzerland) Switzerland), 2014-08, Vol.19 (9), p.13212-13224
Main Authors: Li, Hong Mei, Hwang, Seung Hwan, Kang, Beom Goo, Hong, Jae Seung, Lim, Soon Sung
Format: Article
Language:English
Subjects:
Aldehyde Reductase - antagonists & inhibitors
Aldehyde Reductase - metabolism
aldose reductase
Animals
antioxidant
Antioxidants
Colocasia - chemistry
Colocasia esculenta (L.) Schott
Diabetes
diabetic complication
Enzymes
Ethanol
Herbal medicine
Rats
Rats, Sprague-Dawley
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Summary:The goal of this study was to determine the rat lens aldose reductase-inhibitory effects of 95% ethanol extracts from the leaves of C. esculenta and, its organic solvent soluble fractions, including the dichloromethane (CH2Cl2), ethyl acetate (EtOAc), n-butanol (BuOH) and water (H2O) layers, using dl-glyceraldehyde as a substrate. Ten compounds, namely tryptophan (1), orientin (2), isoorientin (3), vitexin (4), isovitexin (5), luteolin-7-O-glucoside (6), luteolin-7-O-rutinoside (7), rosmarinic acid (8), 1-O-feruloyl-d-glucoside (9) and 1-O-caffeoyl-d-glucoside (10) were isolated from the EtOAc and BuOH fractions of C. esculenta. The structures of compounds 1-10 were elucidated by spectroscopic methods and comparison with previous reports. All the isolates were subjected to an in vitro bioassay to evaluate their inhibitory activity against rat lens aldose reductase. Among tested compounds, compounds 2 and 3 significantly inhibited rat lens aldose reductase, with IC50 values of 1.65 and 1.92 μM, respectively. Notably, the inhibitory activity of orientin was 3.9 times greater than that of the positive control, quercetin (4.12 μM). However, the isolated compounds showed only moderate ABTS+ [2,29-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid)] activity. These results suggest that flavonoid derivatives from Colocasia esculenta (L.) Schott represent potential compounds for the prevention and/or treatment of diabetic complications.
ISSN:1420-3049
1420-3049
DOI:10.3390/molecules190913212