Placental expression of glucose transporters GLUT‐1, GLUT‐3, GLUT‐8 and GLUT‐12 in pregnancies complicated by gestational and type 1 diabetes mellitus

• Published in: Journal of diabetes investigation 2022-03, Vol.13 (3), p.560-570
• Main Authors: Stanirowski, Paweł Jan, Szukiewicz, Dariusz, Majewska, Agata, Wątroba, Mateusz, Pyzlak, Michał, Bomba‐Opoń, Dorota, Wielgoś, Mirosław
• Format: Article
• Language: English
• Subjects: Birth weight; Cesarean section; Diabetes; Diabetes mellitus; Diabetes mellitus (insulin dependent); Diabetes Mellitus, Type 1 - metabolism; Diabetes, Gestational - metabolism; Female; Fetuses; Glucose; Glucose - metabolism; Glucose transporter; Humans; Hyperglycemia; In vitro fertilization; Insulin; Isoforms; Metabolism; Morphometry; Original; Placenta; Placenta - metabolism; Polyclonal antibodies; Pregnancy; Pregnancy in Diabetics - metabolism; Proteins; Quantitative analysis; Statistical analysis
• ISSN: 2040-1116; 2040-1124; 2040-1124
• DOI: 10.1111/jdi.13680

ABSTRACT Aims/Introduction The aim of the present study was to evaluate the placental expression of glucose transporters GLUT‐1, GLUT‐3, GLUT‐8 and GLUT‐12 in term pregnancies complicated by well‐controlled gestational (GDM) and type 1 pregestational diabetes mellitus (PGDM). Materials and Methods A total of 103 placental samples were obtained from patients diagnosed with GDM (n = 60), PGDM (n = 20) and a non‐diabetic control group (n = 23). Computer‐assisted quantitative morphometry of stained placental sections was performed to determine the expression of selected GLUT proteins. Results Immunohistochemical techniques used for the identification of GLUT‐1, GLUT‐3, GLUT‐8 and GLUT‐12 revealed the presence of all glucose transporters in the placental tissue. Morphometric evaluation performed for the vascular density‐matched placental samples demonstrated a significant increase in the expression of GLUT‐1 protein in patients with PGDM as compared to GDM and control groups (P