Free radical scavenging activity, kinetic behaviour and phytochemical constituents of Aristolochia clematitis L. roots

Nowadays there is an increasing demand of natural antioxidants. Imbalanced production and consumption of reactive oxygen species, lead to many diseases such as cancer, arteriosclerosis and ageing processes. The protective effects of plants have long been attributed to their antioxidant compounds, li...

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Bibliographic Details
Published in:Arabian journal of chemistry 2017-02, Vol.10 (S1), p.S1402-S1408
Main Authors: Benmehdi, Houcine, Behilil, Asma, Memmou, Faïza, Amrouche, Abdelillah
Format: Article
Language:English
Subjects:
Antioxidant activity
Aristlochia clematitis L
DPPH
Extracts
Kinetic behaviour
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Summary:Nowadays there is an increasing demand of natural antioxidants. Imbalanced production and consumption of reactive oxygen species, lead to many diseases such as cancer, arteriosclerosis and ageing processes. The protective effects of plants have long been attributed to their antioxidant compounds, like polyphenols, flavonoids, carotenoids, and vitamins C and E. Therefore, the present study was designed to evaluate the antioxidant activity of tannins and crude methanolic extracts of Aristolochia clematitis L. by two complementary test systems (DPPH free radical scavenging and bioautography HPTLC). Besides, phytochemical screening was carried out on roots of A. clematitis and showed the presence of tannins, alkaloids and essential oils. High performance thin layer chromatography (HPTLC) screening provided qualitatively the antioxidant effect of extracts under study. Furthermore, it was found that the tannins and methanolic crude extracts from A. clematitis had a potent DPPH scavenging potency with IC50 values of 0.196 and 0.142mg/mL, respectively. Besides, the kinetic behaviour of DPPH radical scavenging activity of extracts under study allowed us to determine the half life t1/2, time reaction (t) and the remaining DPPH percent.
ISSN:1878-5352
1878-5379
DOI:10.1016/j.arabjc.2013.04.015