A validated method using RP-HPLC for quantification of reserpine in the Brazilian tree Rauvolfia sellowii Müll. Arg. (Apocynaceae)

This study describes a simple, fast and reproducible method using RP-HPLC-UV, in a gradient system, for quantification of reserpine in Rauvolfia sellowii stem bark. The analysis were carried out on a C18 column; mobile phase was water and acetonitrile, and separations were carried out in 10 min, flo...

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Bibliographic Details
Published in:Química Nova 2012, Vol.35 (2), p.408-410
Main Authors: Baratto, Leopoldo C., Campos, Francinete Ramos, Pontarolo, Roberto, Santos, Cid A. de Moraes
Format: Article
Language:English
Subjects:
CHEMISTRY, MULTIDISCIPLINARY
indole alkaloids
liquid chromatography
quality control and validation
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Summary:This study describes a simple, fast and reproducible method using RP-HPLC-UV, in a gradient system, for quantification of reserpine in Rauvolfia sellowii stem bark. The analysis were carried out on a C18 column; mobile phase was water and acetonitrile, and separations were carried out in 10 min, flow rate of 1.0 mL min-1, 25 ºC and 268 nm. The validation data showed that the method was specific, accurate, precise and robust. Results were linear over a range of 0.625-40.0 μg mL-1, and the mean recovery was 95.1%. The amount of reserpine found in the dried stem bark was 0.01% (m/m).
ISSN:0100-4042
1678-7064
DOI:10.1590/S0100-40422012000200031