Cdc42 Couples T Cell Receptor Endocytosis to GRAF1-Mediated Tubular Invaginations of the Plasma Membrane

T cell activation is immediately followed by internalization of the T cell receptor (TCR). TCR endocytosis is required for T cell activation, but the mechanisms supporting removal of TCR from the cell surface remain incompletely understood. Here we report that TCR endocytosis is linked to the clathr...

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Bibliographic Details
Published in:Cells (Basel, Switzerland) Switzerland), 2019-11, Vol.8 (11), p.1388
Main Authors: Rossatti, Pascal, Ziegler, Luca, Schregle, Richard, Betzler, Verena M, Ecker, Manuela, Rossy, Jérémie
Format: Article
Language:English
Subjects:
cdc42
Cdc42 protein
Cell activation
Cell surface
Cholera
Clathrin
clathrin-independent endocytosis
clic
Cold
Endocytosis
graf1
Immunology
Internalization
Invaginations
Lymphocytes
Lymphocytes T
Microscopy
Morphology
Plasma
Polymerization
t cell receptor
T cell receptors
Transferrins
Tubules
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Summary:T cell activation is immediately followed by internalization of the T cell receptor (TCR). TCR endocytosis is required for T cell activation, but the mechanisms supporting removal of TCR from the cell surface remain incompletely understood. Here we report that TCR endocytosis is linked to the clathrin-independent carrier (CLIC) and GPI-enriched endocytic compartments (GEEC) endocytic pathway. We show that unlike the canonical clathrin cargo transferrin or the adaptor protein Lat, internalized TCR accumulates in tubules shaped by the small GTPase Cdc42 and the Bin/amphiphysin/Rvs (BAR) domain containing protein GRAF1 in T cells. Preventing GRAF1-positive tubules to mature into endocytic vesicles by expressing a constitutively active Cdc42 impairs the endocytosis of TCR, while having no consequence on the uptake of transferrin. Together, our data reveal a link between TCR internalization and the CLIC/GEEC endocytic route supported by Cdc42 and GRAF1.
ISSN:2073-4409
2073-4409
DOI:10.3390/cells8111388