CD34 immunoexpression in penile carcinoma

• Published in: Acta cirúrgica brasileira 2002, Vol.17 (suppl 3), p.12-14
• Main Authors: Martins, Antonio Carlos Pereira, Britto, Sérgio, Takata, Clécio, Tucci Jr, Silvio, Borelli-Bovo, Tiago José, Dias Neto, José Anastácio
• Format: Article
• Language: English
• Subjects: CD34; Penile carcinoma; squamous cell carcinoma of the penis
• ISSN: 0102-8650; 0102-8650; 1678-2674
• DOI: 10.1590/S0102-86502002000900003

OBJECTIVE: To investigate microvessel density as a risk factor in squamous cell carcinoma of the penis. METHODS: Fifty patients with penile carcinoma were evaluated retrospectively. The mean age and standard deviation were 60.8±11.8 years. All of them were treated by penectomy and with positive nodes underwent groin lymphadenectomy. Tumor grading was 36 G1 and 24 G2/3. Primary lesion stage was 22 pT1 and 28 pT2-4. Positive inguinal nodes were observed in 18 patients. Selected paraffin embedded sections were submitted to CD34 immunohistochemical analysis by the avidin-biotin-immunoperoxidase method with antigen retrieval. All slides were examined using an automatic analyzer system and the number of micro-vessels in 10 high magnification power fields (400X) were counted in a blind analysis. RESULTS: Median number of microvessels was 631 in G1 versus 695 in G2/3 tumors (p=0.78), and 696 in pT1 versus 566 pT2-4 tumors (p=0.23). The respective data for pN0 patients was 525 and for pN+ was 696 (p=0.01), which is an unexpected result. CONCLUSION: CD34 immunoexpression or microvessel density determined by this method bear no association with tumor grade, stage or prognosis.