New insights on collagen structural organization and spatial distribution around dental implants: a comparison between machined and laser-treated surfaces

• Published in: Journal of translational medicine 2024-01, Vol.22 (1), p.120-13, Article 120
• Main Authors: Belloni, Alessia, Argentieri, Giulio, Orilisi, Giulia, Notarstefano, Valentina, Giorgini, Elisabetta, D'Addazio, Gianmaria, Orsini, Giovanna, Caputi, Sergio, Sinjari, Bruna
• Format: Article
• Language: English
• Subjects: Biopsy; Care and treatment; Cell adhesion & migration; Collagen; Control; Dental Implants; Dental prosthetics; Diagnosis; Ethanol; Fourier Transform InfraRed Imaging spectroscopy; Fourier transforms; Gingiva - pathology; Gum disease; Healing abutments; Health aspects; Humans; Identification and classification; Implant dentures; Implant surface; Lasers; Microbial mats; Oral hygiene; Osseointegration; Periodontitis; Protein structure; Risk factors; Secondary structure; Spatial distribution; Surface Properties; Surgery; Synthegra; Topography; Wound healing
• ISSN: 1479-5876; 1479-5876
• DOI: 10.1186/s12967-024-04906-4

One of the main factors for the osseointegration of dental implants is the development of an adequate soft tissue barrier, mainly composed by collagen, which protects the implant from bacterial development. The structural features of the peri-implant collagen are influenced by the implant components and, in particular, by the type of the surface. In the clinical practice, healing abutments are characterized by smooth surfaces, named machined. Recently, a new laser technique, Synthegra, has been developed to obtain a topography-controlled surface with micrometric regular pores that seems reducing the risk of peri-implantitis. Based on this background, this study aims investigating the structural organization and spatial distribution of collagen surrounding healing abutments characterized by laser-treated and machined surfaces. Gingiva portions surrounding custom-made healing abutments (HA), characterized by alternated laser-treated and machined surfaces, were collected and analyzed by combining Fourier Transform InfraRed Imaging (FTIRI) spectroscopy, a non-invasive and high-resolution bidimensional analytical technique, with histological and multivariate analyses. Masson's trichrome staining, specific for collagen, highlighted a massive presence of collagen in all the analyzed samples, evidencing a surface-related spatial distribution. The nature of collagen, investigated by the FTIRI spectroscopy, appeared more abundant close to the laser-treated surface, with a perpendicular disposition of the bundles respect to the HA; conversely, a parallel distribution was observed around the machined surface. A different secondary structure was also found, with a higher amount of triple helices and a lower quantity of random coils in collagen close to the laser treated surfaces. FTIRI spectroscopy demonstrates that the use of a laser treated transmucosal surface can improve the morphological organization of the peri-implant collagen, which presents a distribution more similar to that of natural teeth. This trial is registered with ClinicalTrials.gov Identifier: (Registration Number: NCT05754970). Registered 06/03/2023, retrospectively registered, https://clinicaltrials.gov/show/NCT05754970 .