SEM and FT-MIR Analysis of Human Demineralized Dentin Matrix: An In Vitro Study

Recently, the demineralized dentin matrix has been suggested as an alternative material to autologous bone grafts and xenografts for clinical purposes. The aim of this study was to investigate the effect of different times of demineralization on the chemical composition and the surface morphology of...

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Bibliographic Details
Published in:Applied sciences 2022-02, Vol.12 (3), p.1480
Main Authors: Memè, Lucia, Strappa, Enrico M., Monterubbianesi, Riccardo, Bambini, Fabrizio, Mummolo, Stefano
Format: Article
Language:English
Subjects:
Autografts
bone graft
Bone grafts
Bones
Chemical composition
Demineralization
demineralized dentin matrix
Dentin
Ethylenediaminetetraacetic acids
FT-MIR
Growth factors
human
human demineralized dentin matrix
Infrared analysis
Infrared spectra
Infrared spectroscopy
Mineralization
Morphology
Physiology
Proteins
Scanning electron microscopy
SEM
Skin & tissue grafts
Spectrum analysis
Statistical analysis
Substitute bone
Teeth
Tubules
Variance analysis
Xenografts
Xenotransplantation
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Summary:Recently, the demineralized dentin matrix has been suggested as an alternative material to autologous bone grafts and xenografts for clinical purposes. The aim of this study was to investigate the effect of different times of demineralization on the chemical composition and the surface morphology of dentinal particles. Extracted teeth were ground and divided into 5 groups based on demineralization time (T0 = 0 min, T2 = 2 min, T5 = 5 min, T10 = 10 min, and T60 = 60 min) with 12% EDTA. The analysis was performed using Fourier-Transform Mid-Infrared spectroscopy (FT-MIR) and Scanning Electron Microscopy (SEM) (p < 0.05). The FT-MIR analysis showed a progressive reduction of the concentration of both PO43− and CO32− in the specimens (T0 > T2 > T5 > T10 > T60). On the contrary, the organic (protein) component did not undergo any change. The SEM examination showed that increasing the times of demineralization resulted in a smoother surface of the dentin particles and a higher number of dentinal tubules.
ISSN:2076-3417
2076-3417
DOI:10.3390/app12031480