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SEM and FT-MIR Analysis of Human Demineralized Dentin Matrix: An In Vitro Study
Recently, the demineralized dentin matrix has been suggested as an alternative material to autologous bone grafts and xenografts for clinical purposes. The aim of this study was to investigate the effect of different times of demineralization on the chemical composition and the surface morphology of...
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Published in: | Applied sciences 2022-02, Vol.12 (3), p.1480 |
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description | Recently, the demineralized dentin matrix has been suggested as an alternative material to autologous bone grafts and xenografts for clinical purposes. The aim of this study was to investigate the effect of different times of demineralization on the chemical composition and the surface morphology of dentinal particles. Extracted teeth were ground and divided into 5 groups based on demineralization time (T0 = 0 min, T2 = 2 min, T5 = 5 min, T10 = 10 min, and T60 = 60 min) with 12% EDTA. The analysis was performed using Fourier-Transform Mid-Infrared spectroscopy (FT-MIR) and Scanning Electron Microscopy (SEM) (p < 0.05). The FT-MIR analysis showed a progressive reduction of the concentration of both PO43− and CO32− in the specimens (T0 > T2 > T5 > T10 > T60). On the contrary, the organic (protein) component did not undergo any change. The SEM examination showed that increasing the times of demineralization resulted in a smoother surface of the dentin particles and a higher number of dentinal tubules. |
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The aim of this study was to investigate the effect of different times of demineralization on the chemical composition and the surface morphology of dentinal particles. Extracted teeth were ground and divided into 5 groups based on demineralization time (T0 = 0 min, T2 = 2 min, T5 = 5 min, T10 = 10 min, and T60 = 60 min) with 12% EDTA. The analysis was performed using Fourier-Transform Mid-Infrared spectroscopy (FT-MIR) and Scanning Electron Microscopy (SEM) (p < 0.05). The FT-MIR analysis showed a progressive reduction of the concentration of both PO43− and CO32− in the specimens (T0 > T2 > T5 > T10 > T60). On the contrary, the organic (protein) component did not undergo any change. The SEM examination showed that increasing the times of demineralization resulted in a smoother surface of the dentin particles and a higher number of dentinal tubules.</description><identifier>ISSN: 2076-3417</identifier><identifier>EISSN: 2076-3417</identifier><identifier>DOI: 10.3390/app12031480</identifier><language>eng</language><publisher>Basel: MDPI AG</publisher><subject>Autografts ; bone graft ; Bone grafts ; Bones ; Chemical composition ; Demineralization ; demineralized dentin matrix ; Dentin ; Ethylenediaminetetraacetic acids ; FT-MIR ; Growth factors ; human ; human demineralized dentin matrix ; Infrared analysis ; Infrared spectra ; Infrared spectroscopy ; Mineralization ; Morphology ; Physiology ; Proteins ; Scanning electron microscopy ; SEM ; Skin & tissue grafts ; Spectrum analysis ; Statistical analysis ; Substitute bone ; Teeth ; Tubules ; Variance analysis ; Xenografts ; Xenotransplantation</subject><ispartof>Applied sciences, 2022-02, Vol.12 (3), p.1480</ispartof><rights>2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). 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The aim of this study was to investigate the effect of different times of demineralization on the chemical composition and the surface morphology of dentinal particles. Extracted teeth were ground and divided into 5 groups based on demineralization time (T0 = 0 min, T2 = 2 min, T5 = 5 min, T10 = 10 min, and T60 = 60 min) with 12% EDTA. The analysis was performed using Fourier-Transform Mid-Infrared spectroscopy (FT-MIR) and Scanning Electron Microscopy (SEM) (p < 0.05). The FT-MIR analysis showed a progressive reduction of the concentration of both PO43− and CO32− in the specimens (T0 > T2 > T5 > T10 > T60). On the contrary, the organic (protein) component did not undergo any change. The SEM examination showed that increasing the times of demineralization resulted in a smoother surface of the dentin particles and a higher number of dentinal tubules.</description><subject>Autografts</subject><subject>bone graft</subject><subject>Bone grafts</subject><subject>Bones</subject><subject>Chemical composition</subject><subject>Demineralization</subject><subject>demineralized dentin matrix</subject><subject>Dentin</subject><subject>Ethylenediaminetetraacetic acids</subject><subject>FT-MIR</subject><subject>Growth factors</subject><subject>human</subject><subject>human demineralized dentin matrix</subject><subject>Infrared analysis</subject><subject>Infrared spectra</subject><subject>Infrared spectroscopy</subject><subject>Mineralization</subject><subject>Morphology</subject><subject>Physiology</subject><subject>Proteins</subject><subject>Scanning electron microscopy</subject><subject>SEM</subject><subject>Skin & tissue grafts</subject><subject>Spectrum analysis</subject><subject>Statistical analysis</subject><subject>Substitute bone</subject><subject>Teeth</subject><subject>Tubules</subject><subject>Variance analysis</subject><subject>Xenografts</subject><subject>Xenotransplantation</subject><issn>2076-3417</issn><issn>2076-3417</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>PIMPY</sourceid><sourceid>DOA</sourceid><recordid>eNpNkV9LwzAUxYMoOOae_AIBH6WaNH-a-jamc4WNgZu-hqRJJKNra9KC89Nbncjuyz33cvhduAeAa4zuCMnRvWpbnCKCqUBnYJSijCeE4uz8RF-CSYw7NFSOicBoBNabpxVUtYHzbbIqXuC0VtUh-ggbBxf9XtXw0e59bYOq_Jc1w1R3voYr1QX_-TDYYVHDN9-FBm663hyuwIVTVbSTvz4Gr_On7WyRLNfPxWy6TErCaZcoS51AGJdMc6EzwQ0VRGiOnaEWMz4ojZxJFXU8Y4QalubC2pxkuSsd02QMiiPXNGon2-D3Khxko7z8XTThXarQ-bKy0mmXsTzFKtWGKq1zZrDlTCAjDOaEDKybI6sNzUdvYyd3TR-GR0SZcsJxigXKBtft0VWGJsZg3f9VjORPAPIkAPIN7gt1Xg</recordid><startdate>20220201</startdate><enddate>20220201</enddate><creator>Memè, Lucia</creator><creator>Strappa, Enrico M.</creator><creator>Monterubbianesi, Riccardo</creator><creator>Bambini, Fabrizio</creator><creator>Mummolo, Stefano</creator><general>MDPI AG</general><scope>AAYXX</scope><scope>CITATION</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0002-3566-0434</orcidid><orcidid>https://orcid.org/0000-0002-0441-3596</orcidid><orcidid>https://orcid.org/0000-0002-5762-6851</orcidid><orcidid>https://orcid.org/0000-0003-4371-900X</orcidid><orcidid>https://orcid.org/0000-0002-3419-665X</orcidid></search><sort><creationdate>20220201</creationdate><title>SEM and FT-MIR Analysis of Human Demineralized Dentin Matrix: An In Vitro Study</title><author>Memè, Lucia ; Strappa, Enrico M. ; Monterubbianesi, Riccardo ; Bambini, Fabrizio ; Mummolo, Stefano</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c364t-ae4f8011c5b68b786d4838b61fd4e156b61b0fd2a4f67534d5298ee9379fcf5b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Autografts</topic><topic>bone graft</topic><topic>Bone grafts</topic><topic>Bones</topic><topic>Chemical composition</topic><topic>Demineralization</topic><topic>demineralized dentin matrix</topic><topic>Dentin</topic><topic>Ethylenediaminetetraacetic acids</topic><topic>FT-MIR</topic><topic>Growth factors</topic><topic>human</topic><topic>human demineralized dentin matrix</topic><topic>Infrared analysis</topic><topic>Infrared spectra</topic><topic>Infrared spectroscopy</topic><topic>Mineralization</topic><topic>Morphology</topic><topic>Physiology</topic><topic>Proteins</topic><topic>Scanning electron microscopy</topic><topic>SEM</topic><topic>Skin & tissue grafts</topic><topic>Spectrum analysis</topic><topic>Statistical analysis</topic><topic>Substitute bone</topic><topic>Teeth</topic><topic>Tubules</topic><topic>Variance analysis</topic><topic>Xenografts</topic><topic>Xenotransplantation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Memè, Lucia</creatorcontrib><creatorcontrib>Strappa, Enrico M.</creatorcontrib><creatorcontrib>Monterubbianesi, Riccardo</creatorcontrib><creatorcontrib>Bambini, Fabrizio</creatorcontrib><creatorcontrib>Mummolo, Stefano</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>Applied sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Memè, Lucia</au><au>Strappa, Enrico M.</au><au>Monterubbianesi, Riccardo</au><au>Bambini, Fabrizio</au><au>Mummolo, Stefano</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>SEM and FT-MIR Analysis of Human Demineralized Dentin Matrix: An In Vitro Study</atitle><jtitle>Applied sciences</jtitle><date>2022-02-01</date><risdate>2022</risdate><volume>12</volume><issue>3</issue><spage>1480</spage><pages>1480-</pages><issn>2076-3417</issn><eissn>2076-3417</eissn><abstract>Recently, the demineralized dentin matrix has been suggested as an alternative material to autologous bone grafts and xenografts for clinical purposes. The aim of this study was to investigate the effect of different times of demineralization on the chemical composition and the surface morphology of dentinal particles. Extracted teeth were ground and divided into 5 groups based on demineralization time (T0 = 0 min, T2 = 2 min, T5 = 5 min, T10 = 10 min, and T60 = 60 min) with 12% EDTA. The analysis was performed using Fourier-Transform Mid-Infrared spectroscopy (FT-MIR) and Scanning Electron Microscopy (SEM) (p < 0.05). The FT-MIR analysis showed a progressive reduction of the concentration of both PO43− and CO32− in the specimens (T0 > T2 > T5 > T10 > T60). On the contrary, the organic (protein) component did not undergo any change. 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subjects | Autografts bone graft Bone grafts Bones Chemical composition Demineralization demineralized dentin matrix Dentin Ethylenediaminetetraacetic acids FT-MIR Growth factors human human demineralized dentin matrix Infrared analysis Infrared spectra Infrared spectroscopy Mineralization Morphology Physiology Proteins Scanning electron microscopy SEM Skin & tissue grafts Spectrum analysis Statistical analysis Substitute bone Teeth Tubules Variance analysis Xenografts Xenotransplantation |
title | SEM and FT-MIR Analysis of Human Demineralized Dentin Matrix: An In Vitro Study |
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