Isolation and Identification of Type F Bovine Enterovirus from Clinical Cattle with Diarrhoea

Recently, bovine enterovirus (BEV) has caused several respiratory and gastrointestinal diseases outbreaks in cattle. Monitoring the epidemiological and pathogenic characteristics of this virus is crucial to controlling its spread. We isolated a BEV strain with typical cytopathic effects from the fae...

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Bibliographic Details
Published in:Viruses 2021-11, Vol.13 (11), p.2217
Main Authors: Ji, Chengyuan, Zhang, Yao, Sun, Ruini, Ma, Jiale, Pan, Zihao, Yao, Huochun
Format: Article
Language:English
Subjects:
Animals
Antibodies
Binding sites
bovine enterovirus
Cattle
Cattle Diseases - diagnosis
Cattle Diseases - epidemiology
Cattle Diseases - pathology
Cattle Diseases - virology
China
Diarrhea
Diarrhea - diagnosis
Diarrhea - epidemiology
Diarrhea - veterinary
Diarrhea - virology
Disease Outbreaks
Enterovirus Infections - diagnosis
Enterovirus Infections - epidemiology
Enterovirus Infections - pathology
Enterovirus Infections - virology
Enterovirus, Bovine - classification
Enterovirus, Bovine - genetics
Enterovirus, Bovine - isolation & purification
Enteroviruses
Epidemiology
Feces - virology
Female
Gastrointestinal diseases
Genetic analysis
Genome, Viral
Genomes
Growth curves
identification
Infections
isolation
Mice
Mice, Inbred ICR
Microscopy
phylogenetic analysis
Phylogenetics
Phylogeny
Proteins
Sequence Alignment
Transmission electron microscopy
Virulence
Viruses
VP1 protein
Whole Genome Sequencing
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Description
Summary:Recently, bovine enterovirus (BEV) has caused several respiratory and gastrointestinal diseases outbreaks in cattle. Monitoring the epidemiological and pathogenic characteristics of this virus is crucial to controlling its spread. We isolated a BEV strain with typical cytopathic effects from the faeces of cows with significant diarrhoeal symptoms in China and observed the viral particles within 20-30 nm through transmission electron microscopy. Then, we designated this strain as HB19-1 in this study. The multistep growth curves showed that the virus propagated well in the MDBK cells. Molecular genetic analysis of VP1 indicated that HB19-1 belonged to the BEV-F1 group. Although the challenged ICR mice did not exhibit typical disease symptoms in animal infection assay, we observed significant pathological damage in the lungs, intestines, and muscle tissues. In summary, we isolated a BEV strain HB19-1 causing severe diarrhoea in cattle and proposed reinforcing the epidemiological surveillance of this virus.
ISSN:1999-4915
1999-4915
DOI:10.3390/v13112217