Novel CB1-ligands maintain homeostasis of the endocannabinoid system in ω3- and ω6-long-chain-PUFA deficiency

• Published in: Journal of lipid research 2019-08, Vol.60 (8), p.1396-1409
• Main Authors: Hammels, Ina, Binczek, Erika, Schmidt-Soltau, Inga, Jenke, Britta, Thomas, Andreas, Vogel, Matthias, Thevis, Mario, Filipova, Dilyana, Papadopoulos, Symeon, Stoffel, Wilhelm
• Format: Article
• Language: English
• Subjects: 20:35,11,14-endocannabinoids; Animals; arachidonic acid; cannabinoid receptor 1; diet effects; endocannabinoid system-orexin-circuitry; Endocannabinoids - genetics; Endocannabinoids - metabolism; fatty acid desaturase 2-deficient mouse model; Fatty Acid Desaturases - deficiency; Fatty Acids, Omega-3 - deficiency; Fatty Acids, Omega-3 - pharmacology; Fatty Acids, Omega-6 - deficiency; Fatty Acids, Omega-6 - pharmacology; HEK293 Cells; Humans; lipid metabolism; Mice; Mice, Knockout; polyunsaturated fatty acid; Receptor, Cannabinoid, CB1 - agonists; Receptor, Cannabinoid, CB1 - genetics; Receptor, Cannabinoid, CB1 - metabolism; Receptor, Cannabinoid, CB2 - agonists; Receptor, Cannabinoid, CB2 - genetics; Receptor, Cannabinoid, CB2 - metabolism; surrogate cannabinoid receptor 1 ligands; ω3 fatty acids
• ISSN: 0022-2275; 1539-7262
• DOI: 10.1194/jlr.M094664

Mammalian ω3- and ω6-PUFAs are synthesized from essential fatty acids (EFAs) or supplied by the diet. PUFAs are constitutive elements of membrane architecture and precursors of lipid signaling molecules. EFAs and long-chain (LC)-PUFAs are precursors in the synthesis of endocannabinoid ligands of Gi/o protein-coupled cannabinoid receptor (CB)1 and CB2 in the endocannabinoid system, which critically regulate energy homeostasis as the metabolic signaling system in hypothalamic neuronal circuits and behavioral parameters. We utilized the auxotrophic fatty acid desaturase 2-deficient (fads2−/−) mouse, deficient in LC-PUFA synthesis, to follow the age-dependent dynamics of the PUFA pattern in the CNS-phospholipidome in unbiased dietary studies of three cohorts on sustained LC-PUFA-free ω6-arachidonic acid- and DHA-supplemented diets and their impact on the precursor pool of CB1 ligands. We discovered the transformation of eicosa-all cis-5,11,14-trienoic acid, uncommon in mammalian lipidomes, into two novel endocannabinoids, 20:35,11,14-ethanolamide and 2-20:35,11,14-glycerol. Their function as ligands of CB1 has been characterized in HEK293 cells. Labeling experiments excluded Δ8-desaturase activity and proved the position specificity of FADS2. The fads2−/− mutant might serve as an unbiased model in vivo in the development of novel CB1 agonists and antagonists.