Understanding the role of soluble proteins and exosomes in non-invasive urine-based diagnosis of preeclampsia

Preeclampsia is a hypertensive disorder of pregnancy that can lead to stillbirth and preterm birth if not treated promptly. Currently, the diagnosis of preeclampsia relies on clinical symptoms such as hypertension and proteinuria, along with invasive blood tests. Here, we investigate the role of sol...

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Bibliographic Details
Published in:Scientific reports 2024-10, Vol.14 (1), p.24117-10, Article 24117
Main Authors: Kim, Taewoon, Choodinatha, Harshitha Kallubhavi, Kim, Kwang Sik, Shin, Kyusoon, Kim, Hyeon Ji, Park, Jee Yoon, Hong, Jong Wook, Lee, Luke P.
Format: Article
Language:English
Subjects:
631/1647/2230/1467
692/53/2421
Adult
Biomarkers - urine
Blood
Blood tests
CD63 antigen
Diagnosis
DNA-Binding Proteins
Endosomal Sorting Complexes Required for Transport - urine
Exosomes
Exosomes - metabolism
Female
Humanities and Social Sciences
Humans
Hypertension
Kinases
multidisciplinary
Non-invasive diagnosis
Placenta growth factor
Placenta Growth Factor - urine
Pre-eclampsia
Pre-Eclampsia - diagnosis
Pre-Eclampsia - urine
Preeclampsia
Pregnancy
Pregnancy complications
Premature birth
Proteins
Proteinuria
Science
Science (multidisciplinary)
SFlt-1/PlGF ratio
Tetraspanin 30 - metabolism
Tetraspanin 30 - urine
Transcription Factors
Urinary exosome
Urine
Vascular Endothelial Growth Factor Receptor-1 - urine
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Description
Summary:Preeclampsia is a hypertensive disorder of pregnancy that can lead to stillbirth and preterm birth if not treated promptly. Currently, the diagnosis of preeclampsia relies on clinical symptoms such as hypertension and proteinuria, along with invasive blood tests. Here, we investigate the role of soluble proteins and exosomes in noninvasive diagnosing preeclampsia non-invasively using maternal urine and urine-derived exosomes. We quantified the levels of particles and the presence of TSG101 and CD63 in urine and urinary exosomes via the biologically intact exosome separation technology (BEST) platform. Then, we obtained higher levels of soluble proteins such as fms-like tyrosine kinase-1 (sFlt-1) and placental growth factor (PlGF) from urine as it was than urinary exosomes. Compared to commercial blood tests, the sensitivity of the sFlt-1/PlGF ratio was found to be 4.0 times higher in urine tests and 1.5 times higher in tests utilizing urine-derived exosomes. Our findings offer promising possibilities for the early and non-invasive identification of high-risk individuals at risk of preeclampsia, allowing for comprehensive preventive management.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-024-75080-2