Physalis alkekengi L. Extract Reduces the Oxidative Stress, Inflammation and Apoptosis in Endothelial Vascular Cells Exposed to Hyperglycemia

• Published in: Molecules (Basel, Switzerland) Switzerland), 2020-08, Vol.25 (16), p.3747
• Main Authors: Vicas, Laura Gratiela, Jurca, Tunde, Baldea, Ioana, Filip, Gabriela Adriana, Olteanu, Diana, Clichici, Simona Valeria, Pallag, Annamaria, Marian, Eleonora, Micle, Otilia, Crivii, Carmen Bianca, Suciu, Tudor, Craciun, Izabela, Gligor, Felicia Gabriela, Muresan, Mariana
• Format: Article
• Language: English
• Subjects: Acids; Adipocytes; Anti-Inflammatory Agents - pharmacology; Antioxidants; Antioxidants - pharmacology; Apoptosis; Biological activity; Biological effects; Caspase-3; Cell adhesion & migration; Cell cycle; Cytokines; Diabetes; Diabetes mellitus; Endothelial cells; Endothelial Cells - drug effects; Endothelial Cells - metabolism; Endothelial Cells - pathology; Enzymes; Flavonoids; Fruits; Glucose; Growth factors; High-performance liquid chromatography; Humans; Hyperglycemia; Hyperglycemia - physiopathology; Inflammation; Inflammation - drug therapy; Insulin resistance; Interleukin 10; Interleukin 6; Kinases; Lipid peroxidation; Lipids; Liquid chromatography; Malondialdehyde; NF-κB protein; Oxidative stress; Oxidative Stress - drug effects; Permeability; Peroxidation; Phenolic compounds; Phenols; Physalis - chemistry; Physalis alkekengi; Physalis extract; Phytochemicals; Phytotherapy; Plant Extracts - pharmacology; Polyphenols; Proteins; Secretions; Transcription factors; Tumor necrosis factor-TNF; Tumor necrosis factor-α; Umbilical vein
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules25163747

To find new natural remedies in diabetes, this study investigated the biological activity of two extracts obtained from the fruits (PhyF) and herba (PhyH) of L. on human umbilical vein endothelial cells (HUVECs) exposed to normo- and hyperglycemic conditions. The biological effect was quantified by malondialdehyde, IL-31 and IL-33 levels in correlation with physico-chemical characterization and antioxidant activity. Additionally, from PhyP extract, the caspase-3, IL-6, IL-10, tumor necrosis factor (TNF)-α and nuclear transcription factor NFkB expressions were evaluated. HPLC analysis revealed a significant number of phenolic compounds, especially in PhyF extract, with a good antioxidant activity as highlighted by TEAC, CUPRAC or DPPH methods. On HUVECS cells, the extracts were not toxic even at high concentrations. Particularly PhyF extract, diminished lipid peroxidation and inhibited the IL-31 and IL-33 secretions induced by hyperglycemia. The inhibitory effect on proinflammatory cytokines was noticed after both doses of PhyF extract in parallel with the upregulation of anti-inflammatory cytokine IL-10. Moreover, PhyF, especially in a low dose, reduced caspase-3 active form. These experimental findings suggest that Physalis fruits extract exerted beneficial effects in hyperglycemia by inhibition of oxidative stress, inflammation and apoptosis being a good adjuvant option in diabetes.