Importance of macrophage cholesterol content on the flux of cholesterol mass

Net flux of cholesterol represents the difference between efflux and influx and can result in net cell-cholesterol accumulation, net cell-cholesterol depletion, or no change in cellular cholesterol content. We measured radiolabeled cell-cholesterol efflux and cell-cholesterol mass using cholesterol-...

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Bibliographic Details
Published in:Journal of lipid research 2010-11, Vol.51 (11), p.3243-3249
Main Authors: Sankaranarayanan, Sandhya, de la Llera-Moya, Margarita, Drazul-Schrader, Denise, Asztalos, Bela F., Weibel, Ginny L., Rothblat, George H.
Format: Article
Language:English
Subjects:
Animals
Cell Line
Cholesterol - blood
Cholesterol - metabolism
cholesterol-enriched
efflux
Humans
influx
Macrophages, Peritoneal - metabolism
Mice
Reproducibility of Results
reverse cholesterol transport
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Summary:Net flux of cholesterol represents the difference between efflux and influx and can result in net cell-cholesterol accumulation, net cell-cholesterol depletion, or no change in cellular cholesterol content. We measured radiolabeled cell-cholesterol efflux and cell-cholesterol mass using cholesterol-normal and -enriched J774 and elicited mouse peritoneal macrophage cells. Net cell-cholesterol effluxes were observed when cholesterol-enriched J774 cells were incubated with 3.5% apolipoprotein (apo) B depleted human serum, HDL3, and apo A-I. Net cell-cholesterol influxes were observed when cholesterol-normal J774 cells were incubated with the same acceptors except apo A-I. When incubated with 2.5% individual sera, cholesterol mass efflux in free cholesterol (FC)-enriched J774 cells correlated with the HDL-cholesterol (HDL-C) concentrations (r2= 0.4; P=0.003), whereas cholesterol mass influx in cholesterol-normal J774 cells correlated with the LDL cholesterol (LDL-C) concentrations (r2= 0.6; P
ISSN:0022-2275
1539-7262
DOI:10.1194/jlr.M008441