Importance of macrophage cholesterol content on the flux of cholesterol mass

Net flux of cholesterol represents the difference between efflux and influx and can result in net cell-cholesterol accumulation, net cell-cholesterol depletion, or no change in cellular cholesterol content. We measured radiolabeled cell-cholesterol efflux and cell-cholesterol mass using cholesterol-...

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Published in:Journal of lipid research 2010-11, Vol.51 (11), p.3243-3249
Main Authors: Sankaranarayanan, Sandhya, de la Llera-Moya, Margarita, Drazul-Schrader, Denise, Asztalos, Bela F., Weibel, Ginny L., Rothblat, George H.
Format: Article
Language:English
Subjects:
Animals
Cell Line
Cholesterol - blood
Cholesterol - metabolism
cholesterol-enriched
efflux
Humans
influx
Macrophages, Peritoneal - metabolism
Mice
Reproducibility of Results
reverse cholesterol transport
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cited_by cdi_FETCH-LOGICAL-c548t-f171de970fddd5441da18f21cc117bf693e1e04df6d6eb6e76b834e6d32beeeb3
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container_title Journal of lipid research
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creator Sankaranarayanan, Sandhya
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Rothblat, George H.
description Net flux of cholesterol represents the difference between efflux and influx and can result in net cell-cholesterol accumulation, net cell-cholesterol depletion, or no change in cellular cholesterol content. We measured radiolabeled cell-cholesterol efflux and cell-cholesterol mass using cholesterol-normal and -enriched J774 and elicited mouse peritoneal macrophage cells. Net cell-cholesterol effluxes were observed when cholesterol-enriched J774 cells were incubated with 3.5% apolipoprotein (apo) B depleted human serum, HDL3, and apo A-I. Net cell-cholesterol influxes were observed when cholesterol-normal J774 cells were incubated with the same acceptors except apo A-I. When incubated with 2.5% individual sera, cholesterol mass efflux in free cholesterol (FC)-enriched J774 cells correlated with the HDL-cholesterol (HDL-C) concentrations (r2= 0.4; P=0.003), whereas cholesterol mass influx in cholesterol-normal J774 cells correlated with the LDL cholesterol (LDL-C) concentrations (r2= 0.6; P
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We measured radiolabeled cell-cholesterol efflux and cell-cholesterol mass using cholesterol-normal and -enriched J774 and elicited mouse peritoneal macrophage cells. Net cell-cholesterol effluxes were observed when cholesterol-enriched J774 cells were incubated with 3.5% apolipoprotein (apo) B depleted human serum, HDL3, and apo A-I. Net cell-cholesterol influxes were observed when cholesterol-normal J774 cells were incubated with the same acceptors except apo A-I. When incubated with 2.5% individual sera, cholesterol mass efflux in free cholesterol (FC)-enriched J774 cells correlated with the HDL-cholesterol (HDL-C) concentrations (r2= 0.4; P=0.003), whereas cholesterol mass influx in cholesterol-normal J774 cells correlated with the LDL cholesterol (LDL-C) concentrations (r2= 0.6; P&lt;0.0001) of the individual sera. A positive correlation was observed between measurements of [3H]cholesterol efflux and reductions in cholesterol mass (r2= 0.4; P=0.001) in FC-enriched J774 cells. In conclusion, isotopic efflux measurements from cholesterol-normal or cholesterol-enriched cells provide an accurate measurement of relative ability of an acceptor to remove labeled cholesterol under a specific set of experimental conditions, i.e., efflux potential. 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subjects Animals
Cell Line
Cholesterol - blood
Cholesterol - metabolism
cholesterol-enriched
efflux
Humans
influx
Macrophages, Peritoneal - metabolism
Mice
Reproducibility of Results
reverse cholesterol transport
title Importance of macrophage cholesterol content on the flux of cholesterol mass
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