Two membrane-bound transcription factors regulate expression of various type-IV-pili surface structures in Sulfolobus acidocaldarius

In Archaea and Bacteria, gene expression is tightly regulated in response to environmental stimuli. In the thermoacidophilic crenarchaeon s nutrient limitation induces expression of the archaellum, the archaeal motility structure. This expression is orchestrated by a complex hierarchical network of...

Full description

Saved in:
Bibliographic Details
Published in:PeerJ (San Francisco, CA) CA), 2019-02, Vol.7, p.e6459, Article e6459
Main Authors: Bischof, Lisa Franziska, Haurat, Maria Florencia, Albers, Sonja-Verena
Format: Article
Language:English
Subjects:
Appendages
Archaea
Bacteria
Bacterial genetics
Biochemistry
Biofilms
Biology
Cell motility
Chromosomes
Deoxyribonucleic acid
DNA
DNA binding proteins
E coli
Environmental effects
Gene expression
Genes
Kinases
Membrane protein
Metabolism
Microbiology
Molecular Biology
Motility
Oligomers
One-component system
Phosphatase
Pili
Plasmids
Prokaryotes
Promoters (Genetics)
Protein-serine/threonine kinase
Proteins
Signal transduction
Sulfolobus acidocaldarius
Transcription factors
Transcription regulation
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:In Archaea and Bacteria, gene expression is tightly regulated in response to environmental stimuli. In the thermoacidophilic crenarchaeon s nutrient limitation induces expression of the archaellum, the archaeal motility structure. This expression is orchestrated by a complex hierarchical network of positive and negative regulators-the archaellum regulatory network (arn). The membrane-bound one-component system ArnR and its paralog ArnR1 were recently described as main activators of archaellum expression in . They regulate gene expression of the archaellum operon by targeting the promoter of , encoding the archaellum filament protein. Here we describe a strategy for the isolation and biochemical characterization of these two archaellum regulators. Both regulators are capable of forming oligomers and are phosphorylated by the Ser/Thr kinase ArnC. Apart from binding to , ArnR but not ArnR1 bound to promoter sequences of and , which encode components of the archaeal adhesive pilus and UV-inducible pili system, demonstrating a regulatory connection between different surface appendages of .
ISSN:2167-8359
2167-8359
DOI:10.7717/peerj.6459