Postcolectomy Peritoneal Environment Increases Colon Cancer Cell Migration Capacity

Background. Clinical data and animal models support an association between postoperative inflammatory response and the risk of colorectal cancer recurrence. Our aim was to evaluate postoperative peritoneal inflammation and its impact on cultured colon cancer cells’ migration capacity. Methods. 23 pa...

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Bibliographic Details
Published in:Gastroenterology research and practice 2016-01, Vol.2016 (2016), p.1-6
Main Authors: Avital, Shmuel, White, Ian, Shpitz, Baruch, Majdop, Salem, Ghinea, Ronen, Berkovich, L., Mishaeli, Moshe
Format: Article
Language:English
Subjects:
Abdomen
Care and treatment
Cell migration
Colon cancer
Colorectal cancer
Colorectal surgery
Complications and side effects
Cytokines
Development and progression
Fluids
Health aspects
Inflammation
Medical research
Nonsteroidal anti-inflammatory drugs
Observations
Patients
Postoperative period
Software
Statistical analysis
Studies
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Summary:Background. Clinical data and animal models support an association between postoperative inflammatory response and the risk of colorectal cancer recurrence. Our aim was to evaluate postoperative peritoneal inflammation and its impact on cultured colon cancer cells’ migration capacity. Methods. 23 patients undergoing elective colorectal resection with uneventful recovery were prospectively enrolled. Patients were operated on for both malignant and benign etiologies. Peritoneal fluids collected at surgery initiation and after surgery were evaluated for their effect on migration potential of human colon cancer cells using an in vitro scratch assay and on TNF-α, IL-1β, IL-6, and IL-10 levels using bead-based fluorokine-linked multianalyte profiling. Results. Postoperative peritoneal fluid from all patients increased the migration capacity of colon cancer cells compared to preoperative levels. This effect was significant during the first two postoperative days and decreased thereafter. The increase in colon cancer cell migration capacity correlated with increased levels of peritoneal TNF-α and IL-10. Conclusion. In this pilot study, we have demonstrated that the intraperitoneal environment following colorectal resection significantly enhances colon cancer cells migration capacity. This effect is associated with postoperative intra-abdominal cytokines level. A larger scale study in colorectal cancer patients is needed in order to correlate these findings with perioperative parameters and clinical outcome.
ISSN:1687-6121
1687-630X
DOI:10.1155/2016/2540397