Loading…

Wheat grain biofortification for essential amino acids

Abstract The objective of this work was to select wheat genotypes aiming to increase the essential amino acids in their grains. The study was carried out in the 2019 crop year, in a randomized complete block design, organized in a 5x5 factorial arrangement – five environments in the state of Rio Gra...

Full description

Saved in:
Bibliographic Details
Published in:Pesquisa agropecuaria brasileira 2023-01, Vol.58
Main Authors: Loro, Murilo Vieira, Carvalho, Ivan Ricardo, Cargnelutti Filho, Alberto, Hoffmann, Jessica Fernanda, Kehl, Kassiana
Format: Article
Language:English
Subjects:
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Abstract The objective of this work was to select wheat genotypes aiming to increase the essential amino acids in their grains. The study was carried out in the 2019 crop year, in a randomized complete block design, organized in a 5x5 factorial arrangement – five environments in the state of Rio Grande do Sul, Brazil (Cachoeira do Sul, Cruz Alta, Santo Augusto, São Gabriel, and Vacaria), and five wheat genotypes ('BRS Parrudo', 'Marfim', 'Quartzo', 'TBIO Mestre', and 'TBIO Sinuelo') –, with two replicates. Polar metabolites were extracted from the flour of the ground wheat grains, derivatized, and evaluated by gas chromatography-mass spectrometry. Both variance components and genetic parameters were estimated for the metabolites. To select the genotypes for the traits of interest, the multi-trait index based on factor analysis and ideotype design, the multi-trait genotype-ideotype distance index, and the multi-trait stability index were applied. The wheat genotypes express a high genetic variability and selection possibility for gentiobiose, butyric acid, galactopyranosyl, phenylalanine, tryptophan, leucine, and isoleucine. The 'Marfim' genotype remains stable for essential amino acid levels in the studied environments. The 'Quartzo' genotype stands out in the expression of leucine, isoleucine, phenylalanine, and tryptophan in its grains. Resumo O objetivo deste trabalho foi selecionar genótipos de trigo com vista ao aumento de aminoácidos essenciais nos seus grãos. O estudo foi realizado no ano agrícola de 2019, em delineamento de blocos ao acaso, organizados em arranjo fatorial 5x5 – cinco ambientes no estado do Rio Grande do Sul, Brasil (Cachoeira do Sul, Cruz Alta, Santo Augusto, São Gabriel e Vacaria), e cinco genótipos de trigo ('BRS Parrudo', 'Marfim', 'Quartzo', 'TBIO Mestre' e 'TBIO Sinuelo') –, com duas repetições. Os metabólitos polares foram extraídos da farinha dos grãos de trigo moídos, derivatizados e avaliados por cromatografia gasosa-espectrometria de massa. Tanto os componentes de variância como os parâmetros genéticos foram estimados para os metabólitos. Para selecionar os genótipos para as características de interesse, aplicaram-se o índice multicaracterística baseado em análise fatorial e desenho de ideótipos, o índice de distância genótipo-ideótipo multicaracterística e o índice de estabilidade multicaracterística. Os genótipos de trigo expressam alta variabilidade genética e possibilidade de seleção para gentiobiose, ácido butírico,
ISSN:0100-204X
1678-3921
1678-3921
DOI:10.1590/s1678-3921.pab2023.v58.02860