Lack of APOL1 in proximal tubules of normal human kidneys and proteinuric APOL1 transgenic mouse kidneys

The mechanism of pathogenesis associated with APOL1 polymorphisms and risk for non-diabetic chronic kidney disease (CKD) is not fully understood. Prior studies have minimized a causal role for the circulating APOL1 protein, thus efforts to understand kidney pathogenesis have focused on APOL1 express...

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Bibliographic Details
Published in:PloS one 2021-06, Vol.16 (6), p.e0253197-e0253197
Main Authors: Blessing, Natalya A, Wu, Zhenzhen, Madhavan, Sethu M, Choy, Jonathan W, Chen, Michelle, Shin, Myung K, Hoek, Maarten, Sedor, John R, O'Toole, John F, Bruggeman, Leslie A
Format: Article
Language:English
Subjects:
Alleles
Analysis
Animal models in research
Animals
Apolipoprotein L1 - genetics
Apolipoprotein L1 - metabolism
Biology and Life Sciences
Endothelial Cells - metabolism
Engineering and Technology
Evaluation
Genetic aspects
Genetic engineering
Genetic polymorphisms
Humans
Kidney
Kidney Tubules, Proximal - metabolism
Kidneys
Liver - metabolism
Medicine and Health Sciences
Mice
Mice, Transgenic
Podocytes - metabolism
Proteinuria - genetics
Proteinuria - metabolism
Research and Analysis Methods
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Summary:The mechanism of pathogenesis associated with APOL1 polymorphisms and risk for non-diabetic chronic kidney disease (CKD) is not fully understood. Prior studies have minimized a causal role for the circulating APOL1 protein, thus efforts to understand kidney pathogenesis have focused on APOL1 expressed in renal cells. Of the kidney cells reported to express APOL1, the proximal tubule expression patterns are inconsistent in published reports, and whether APOL1 is synthesized by the proximal tubule or possibly APOL1 protein in the blood is filtered and reabsorbed by the proximal tubule remains unclear. Using both protein and mRNA in situ methods, the kidney expression pattern of APOL1 was examined in normal human and APOL1 bacterial artificial chromosome transgenic mice with and without proteinuria. APOL1 protein and mRNA was detected in podocytes and endothelial cells, but not in tubular epithelia. In the setting of proteinuria, plasma APOL1 protein did not appear to be filtered or reabsorbed by the proximal tubule. A side-by-side examination of commercial antibodies used in prior studies suggest the original reports of APOL1 in proximal tubules likely reflects antibody non-specificity. As such, APOL1 expression in podocytes and endothelia should remain the focus for mechanistic studies in the APOL1-mediated kidney diseases.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0253197