Lack of APOL1 in proximal tubules of normal human kidneys and proteinuric APOL1 transgenic mouse kidneys

• Published in: PloS one 2021-06, Vol.16 (6), p.e0253197-e0253197
• Main Authors: Blessing, Natalya A, Wu, Zhenzhen, Madhavan, Sethu M, Choy, Jonathan W, Chen, Michelle, Shin, Myung K, Hoek, Maarten, Sedor, John R, O'Toole, John F, Bruggeman, Leslie A
• Format: Article
• Language: English
• Subjects: Alleles; Analysis; Animal models in research; Animals; Apolipoprotein L1 - genetics; Apolipoprotein L1 - metabolism; Biology and Life Sciences; Endothelial Cells - metabolism; Engineering and Technology; Evaluation; Genetic aspects; Genetic engineering; Genetic polymorphisms; Humans; Kidney; Kidney Tubules, Proximal - metabolism; Kidneys; Liver - metabolism; Medicine and Health Sciences; Mice; Mice, Transgenic; Podocytes - metabolism; Proteinuria - genetics; Proteinuria - metabolism; Research and Analysis Methods
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0253197

The mechanism of pathogenesis associated with APOL1 polymorphisms and risk for non-diabetic chronic kidney disease (CKD) is not fully understood. Prior studies have minimized a causal role for the circulating APOL1 protein, thus efforts to understand kidney pathogenesis have focused on APOL1 expressed in renal cells. Of the kidney cells reported to express APOL1, the proximal tubule expression patterns are inconsistent in published reports, and whether APOL1 is synthesized by the proximal tubule or possibly APOL1 protein in the blood is filtered and reabsorbed by the proximal tubule remains unclear. Using both protein and mRNA in situ methods, the kidney expression pattern of APOL1 was examined in normal human and APOL1 bacterial artificial chromosome transgenic mice with and without proteinuria. APOL1 protein and mRNA was detected in podocytes and endothelial cells, but not in tubular epithelia. In the setting of proteinuria, plasma APOL1 protein did not appear to be filtered or reabsorbed by the proximal tubule. A side-by-side examination of commercial antibodies used in prior studies suggest the original reports of APOL1 in proximal tubules likely reflects antibody non-specificity. As such, APOL1 expression in podocytes and endothelia should remain the focus for mechanistic studies in the APOL1-mediated kidney diseases.