Three-Dimensional Telomeric Analysis of Isolated Circulating Tumor Cells (CTCs) Defines CTC Subpopulations

Abstract Circulating tumor cells (CTCs) have been identified with the potential to serve as suitable biomarkers for tumor stage and progression, but the availability of effective isolation technique(s) coupled with detailed molecular characterization have been the challenges encountered in making CT...

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Bibliographic Details
Published in:Translational oncology 2013-02, Vol.6 (1), p.51-IN4
Main Authors: Adebayo Awe, Julius, Xu, Mark Chu, Wechsler, Janine, Benali-Furet, Naoual, Cayre, Yvon E, Saranchuk, Jeff, Drachenberg, Darrel, Mai, Sabine
Format: Article
Language:English
Subjects:
architecture
biomarkers
breast-cancer
Cancer
chromosomal rearrangements
interphase
Life Sciences
Medical Biotechnology
Medicinsk bioteknologi
microscopy
Natural sciences
Naturvetenskap
nucleus
Oncology
organization
peripheral-blood
prostate-cancer
survival
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Summary:Abstract Circulating tumor cells (CTCs) have been identified with the potential to serve as suitable biomarkers for tumor stage and progression, but the availability of effective isolation technique(s) coupled with detailed molecular characterization have been the challenges encountered in making CTCs clinically relevant. For the first time, we combined isolation of CTCs using the ScreenCell filtration technique with quantitative analysis of CTC telomeres by TeloView. This resulted in the identification and molecular characterization of different subpopulations of CTCs in the same patient. Three-dimensional (3D) telomeric analysis was carried out on isolated CTCs of 19 patients that consisted of four different tumor types, namely, prostate, colon, breast, melanoma, and one lung cancer cell line. With telomeric analysis of the filter-isolated CTCs, the level of chromosomal instability (CIN) of the CTCs can be determined. Our study shows that subpopulations of CTCs can be identified on the basis of their 3D telomeric properties.
ISSN:1936-5233
1944-7124
1936-5233
DOI:10.1593/tlo.12361