Genome-wide analysis of long noncoding RNA expression profile in nasal mucosa with allergic rhinitis

Long noncoding RNAs (lncRNAs) are involved in a variety of human immune diseases. However, the expression profile and precise function of lncRNAs in allergic rhinitis (AR) remain unknown. In the present study, genome-wide analysis of lncRNA expression was performed in the nasal mucosa tissue and mRN...

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Bibliographic Details
Published in:BMC medical genomics 2021-04, Vol.14 (1), p.100-12, Article 100
Main Authors: Wei, Xian, Xu, Man, Wang, Chao, Fang, Shengjian, Zhang, Yu, Wang, Weihua
Format: Article
Language:English
Subjects:
Adult
Allergens
Allergic rhinitis
Allergies
Cell adhesion
Cell culture
Chemokine CXCL12 - genetics
Chemokine CXCL12 - metabolism
Correlation analysis
CXCL12
CXCL12 protein
CXCR4
CXCR4 protein
Development and progression
DNA microarrays
Expression profile
Female
Gene expression
Gene Expression Profiling
Gene Regulatory Networks
Genes
Genetic aspects
Genome-Wide Association Study
Genomes
Genomics
Hay-fever
Health aspects
Humans
Hypersensitivity
Immunological diseases
Inflammation
Leukocyte migration
Long non-coding RNA
Male
Medical research
Medicine, Experimental
Methods
Microarray
Middle Aged
mRNA
Mucosa
Nasal Mucosa - metabolism
Nasal Mucosa - pathology
Polymerase chain reaction
Receptors, CXCR4 - genetics
Receptors, CXCR4 - metabolism
Rhinitis, Allergic - genetics
Rhinitis, Allergic - metabolism
RNA
RNA, Long Noncoding - genetics
RNA, Messenger - genetics
RNA, Messenger - metabolism
Software
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Description
Summary:Long noncoding RNAs (lncRNAs) are involved in a variety of human immune diseases. However, the expression profile and precise function of lncRNAs in allergic rhinitis (AR) remain unknown. In the present study, genome-wide analysis of lncRNA expression was performed in the nasal mucosa tissue and mRNA regulatory relationship was examined among patients with or without AR. Microarray assays were performed and the differential expressions of lncRNAs or mRNA were verified through RT-PCR. The lncRNA functions were annotated using Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG). The potential regulatory relationships between lncRNAs and the co-expressed mRNAs were analyzed using Cytoscape software. The expressions of specific lncRNAs and mRNAs were examined using an in vitro cell model. A total of 57 lncRNAs and 127 mRNAs were dysregulated in the nasal mucosa tissue of patients with AR, compared to those of patients without AR (fold change > 2.0 and P 
ISSN:1755-8794
1755-8794
DOI:10.1186/s12920-021-00949-4