A Portable Continuous-Flow Polymerase Chain Reaction Chip Device Integrated with Arduino Boards for Detecting Colla corii asini

• Published in: Micromachines (Basel) 2022-08, Vol.13 (8), p.1289
• Main Authors: Sheu, Shyang-Chwen, Song, Yi-Syuan, Chen, Jyh-Jian
• Format: Article
• Language: English
• Subjects: Amplification; Arduino; Boards; Colla corii asini; Conduction heating; Continuous flow; Controllers; Cost analysis; Deoxyribonucleic acid; DNA; Flow velocity; Fluid flow; Food security; Foot & mouth disease; Glass substrates; Heaters; Identification methods; Indium tin oxides; Microelectromechanical systems; Microfluidics; Nucleic acids; Pathogens; Polymerase chain reaction; portable device; Portable equipment; Pumping; Traditional Chinese medicine
• ISSN: 2072-666X; 2072-666X
• DOI: 10.3390/mi13081289

Food security is a significant issue in modern society. Because morphological characters are not reliable enough to distinguish authentic traditional Chinese medicines, it is essential to establish an effective and applicable method to identify them to protect people’s health. Due to the expensive cost of the manufacturing process and the large volume of the analytical system, the need to build a portable and cheap device is urgent. This work describes the development of a portable nucleic acid amplification device integrated with thermal control and liquid pumping connecting to Arduino boards. We present a novel microfluidic polymerase chain reaction (PCR) chip with symmetric isothermal zones. The total chip volume is small, and only one Arduino board is needed for thermal control. We assemble a miniaturized liquid pump and program an Arduino file to push the sample mixture into the chip to implement the PCR process. In the proposed operation, the Nusselt number of the sample flow is less than one, and the heat transfer is conduction only. Then we can ensure temperature uniformity in specific reaction regions. A Colla corii asini DNA segment of 200 bp is amplified to evaluate the PCR performance under the various operational parameters. The initial concentration for accomplishing the PCR process is at least 20 ng/μL at the flow rate of 0.4 μL/min in the portable continuous flow PCR (CFPCR) device. To our knowledge, our group is the first to introduce Arduino boards into the heat control and sample pumping modules for a CFPCR device.