Detection of Oxazolidinone Resistance Genes and Characterization of Genetic Environments in Enterococci of Swine Origin, Italy

One hundred forty-five florfenicol-resistant enterococci, isolated from swine fecal samples collected from 76 pig farms, were investigated for the presence of , , and genes by PCR. Thirty florfenicol-resistant isolates had at least one linezolid resistance gene. was found to be the most widespread l...

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Published in:Microorganisms (Basel) 2020-12, Vol.8 (12), p.2021
Main Authors: Fioriti, Simona, Morroni, Gianluca, Coccitto, Sonia Nina, Brenciani, Andrea, Antonelli, Alberto, Di Pilato, Vincenzo, Baccani, Ilaria, Pollini, Simona, Cucco, Lucilla, Morelli, Alessandra, Paniccià, Marta, Magistrali, Chiara Francesca, Rossolini, Gian Maria, Giovanetti, Eleonora
Format: Article
Language:English
Subjects:
Animals
Antibiotics
Antimicrobial agents
cfr
cfr(D)
Chromosomes
Deoxyribonucleic acid
DNA
DNA methylation
Enterococcus faecalis
Enterococcus faecium
Enterococcus spp
Experiments
Farms
Florfenicol
Genes
Genetic analysis
Hogs
Infections
Linezolid
Mutation
oxazolidinone resistance
Pathogens
Phylogenetics
Plasmids
Swine
Transposons
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Summary:One hundred forty-five florfenicol-resistant enterococci, isolated from swine fecal samples collected from 76 pig farms, were investigated for the presence of , , and genes by PCR. Thirty florfenicol-resistant isolates had at least one linezolid resistance gene. was found to be the most widespread linezolid resistance gene (23/30), while and were detected in 6/30 and 7/30 enterococcal isolates, respectively. WGS analysis also showed the presence of the (D) gene in ( = 2 isolates) and in ( = 1 isolate). The linezolid resistance genes hybridized both on chromosome and plasmids ranging from ~25 to ~240 kb. Twelve isolates were able to transfer linezolid resistance genes to enterococci recipient. WGS analysis displayed a great variability of genetic contexts identical or related to transposons (Tn and Tn ), plasmids (pE035 and pWo27-9), and chromosomal regions. environments showed identities with Tn -like transposon and a region from p12-2300 plasmid; (D) genetic contexts were related to the corresponding region of the plasmid 4 of E8014; was always found on Tn . Circular forms were obtained only for - and -carrying genetic contexts. Clonality analysis revealed the presence of (ST16, ST27, ST476, and ST585) and (ST21) clones previously isolated from humans. These results demonstrate a dissemination of linezolid resistance genes in enterococci of swine origin in Central Italy and confirm the spread of linezolid resistance in animal settings.
ISSN:2076-2607
2076-2607
DOI:10.3390/microorganisms8122021