Optimization of Photoautotrophic Growth Regimens of Scenedesmaceae alga: The Influence of Light Conditions and Carbon Dioxide Concentrations

• Published in: Applied sciences 2023-12, Vol.13 (23), p.12753
• Main Authors: Ziganshina, Elvira E., Bulynina, Svetlana S., Yureva, Ksenia A., Ziganshin, Ayrat M.
• Format: Article
• Language: English
• Subjects: Algae; algal culture; Biomass; Carbon dioxide; Labfors 4 Lux photobioreactor; Light; Metabolism; Nitrates; Nutrient removal; Optimization; photoautotrophic growth; photoperiod; PPFD; Scenedesmaceae
• ISSN: 2076-3417; 2076-3417
• DOI: 10.3390/app132312753

Improving methods for landless production of bioproducts is considered an important stage in the development of the modern bioeconomy. In this context, microalgal biomass is one of the most promising sources of valuable substances due to its rich biochemical composition. Despite the high adaptability of microalgae to various environmental factors, the effectiveness of cultivation systems depends on precisely selected parameters. Both the light conditions and the supply of inorganic carbon sources are key in determining the efficiency of photoautotrophic cultivation. In this work, the effect of a high daily photosynthetic photon flux density (PPFD) ranging from 37.44 to 112.32 mol m−2 day−1 on the growth and productivity of a novel Scenedesmaceae alga, strain EZ-B1, was assessed. The next stage of cultivation consisted of selecting the optimal CO2 concentration. Improved performance of microalga during cultivation in a photobioreactor was achieved at 112.32 mol m−2 day−1 (24 h photoperiod) and by supplying 2% CO2, as evidenced by the high biomass productivity (0.69 g L−1 day−1), total biomass yield (5.23 g L−1), and ammonium nitrogen consumption rate. The data obtained suggest that a higher level of PPFD led to the highest growth rate of the novel strain and the highest biomass productivity, which, in practice, will increase production capacity.