Disease burden, antimicrobial resistance and molecular characterization of invasive group B Streptococcus among non-pregnant adults in Malaysia: A protocol study

Objective: To determine the prevalence of group B Streptococcus (GBS) isolated from non-pregnant adults from seven major hospitals in Peninsular Malaysia; the incidence, case-fatality ratio, antimicrobial susceptibility patterns, serotype, genotype and distribution of the GBS virulence and pili gene...

Full description

Saved in:
Bibliographic Details
Published in:Asian Pacific journal of tropical medicine 2023-11, Vol.16 (11), p.498-505
Main Authors: Muthanna, Abdulrahman, Baharin, Nurul Hana Zainal, Desa, Mohd Nasir Mohd, Dzaraly, Nurul Diana, Azmai, Mohammad Noor Amal, Amin-Nordin, Syafinaz
Format: Article
Language:English
Subjects:
group b streptococcus
incidence
serotype
antimicrobial resistance
virulence gene
multi-locus sequence typing
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Objective: To determine the prevalence of group B Streptococcus (GBS) isolated from non-pregnant adults from seven major hospitals in Peninsular Malaysia; the incidence, case-fatality ratio, antimicrobial susceptibility patterns, serotype, genotype and distribution of the GBS virulence and pili genes are also investigated. Methods: This multicentre, prospective, observational study is conducted in seven major tertiary hospitals in Malaysia among non-pregnant adults. Simultaneously, a retrospective study is conducted in the selected hospitals with similar approaches. GBS isolates are subjected to phenotyping, serotyping by multiplex PCR, antimicrobial susceptibility testing and PCR-detection of GBS virulence and pilus genes. Seven housekeeping genes are amplified and sequenced for multi-locus sequence typing. Discussion: Findings from the study may contribute to the management of clinical practice to diagnose and prevent GBS related diseases in a timely manner. Prudent use of antibiotics is encouraged by monitoring antimicrobial resistance.
ISSN:1995-7645
2352-4146
DOI:10.4103/1995-7645.390165