New Sesquiterpenenoids from Ainsliaea yunnanensis

• Published in: Molecules (Basel, Switzerland) Switzerland), 2016-08, Vol.21 (8), p.1031-1031
• Main Authors: Wu, Xiang-Lei, Xiong, Xiao-Juan, Lu, Wen-Quan, Huang, Hao, Shen, Yun-Heng, Wu, Zhi-Jun, Chen, Wan-Sheng
• Format: Article
• Language: English
• Subjects: Ainsliaea; Ainsliaea yunnanensis; Animals; anti-inflammatory activity; Anti-Inflammatory Agents - chemistry; Anti-Inflammatory Agents - pharmacology; Asteraceae - chemistry; Crystallography, X-Ray; Cytokines - metabolism; Gene Expression Regulation - drug effects; Lactones - chemistry; Lactones - pharmacology; Lipopolysaccharides - adverse effects; Mice; NF-kappa B - antagonists & inhibitors; Pharmacy; RAW 264.7 Cells; Sesquiterpenes - chemistry; Sesquiterpenes - pharmacology; sesquiterpenoid
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules21081031

Investigation of the ethanol extract of the whole plant of Ainsliaea yunnanensis led to the isolation of four new dimeric sesquiterpene lactones, ainsliadimer F-I (1-4), together with seven known dimeric sesquiterpene lactones (5-11) and ten sesquiterpenes (12-21). Their structures were elucidated by spectroscopic methods. The relative stereochemistry of ainsliadimer F was further confirmed by single crystal X-ray diffraction analysis. Compounds 1-21 were tested for the inhibition of nuclear factor kappa B (NF-κB) in the 293-NF-κB-luciferase reporter cell line induced by lipopolysaccharide (LPS), and Compounds 5, 18, 20 and 21 were further tested for the production of TNF-α, IL-1β, IL-6 and IL-10 in RAW 264.7 macrophages induced by LPS. Compounds 5, 18, 20 and 21 exhibited significant activity in anti-inflammatory activity assays.