Forensic significance of cTnI serum for the detection of terminal myocardial damage in rats (Rattus norvegicus) caused by hyperthermia

• Published in: Journal of King Saud University. Science 2022-02, Vol.34 (2), p.101753, Article 101753
• Main Authors: Dervišević, Emina, Hasić, Sabaheta, Katica, Muhamed, Salihbegović, Adis, Ajanović, Zurifa, Sarajlić, Nermin
• Format: Article
• Language: English
• Subjects: Cardiac protein I; Forensic significance; Hyperthermia; Myocardium
• ISSN: 1018-3647
• DOI: 10.1016/j.jksus.2021.101753

Figure 2. Difference in values of repeated measurement of cardiac troponin I in rat serum in experimental groups. The values of the cardiac isoform of troponin I are presented in the form of a median with an interquartile range (25–75 percentile). Vertical lines represent the range of minimum and maximum values. cTnI- cardiac isoform of troponin I (b-basal values; p-repeated measurements); p-probability; KG37-control group of rats exposed to water temperature of 37 °C; G41 group exposed to water temperature 41 °C. [Display omitted] To determine whether the biochemical marker cTnI with circulating value has forensic significance in the detection of myocardial damage in rats in experimental conditions of hyperthermia by exposure to water temperatures of 41 °C. The study was conducted on 24 adult Wistar rats that were divided into two experimental groups, depending on water temperature exposure of 37 °C (KG, n = 8) and 41 °C (G41, n = 16). Depending on the length of time of exposure to water temperatures of 41 °C are further divided into G41-AM (20 min of exposure), G41-PM (exposure until death). Blood for analysis of the concentration of cardiac troponin I (cTnI) was taken before the experiment and after the expiration of the given duration of the experiment of 20 min or death. The concentration of cTnI was determined in serum by ELISA method. The level of statistical significance was, p