Farnesol Inhibits PI3 Kinase Signaling and Inflammatory Gene Expression in Primary Human Renal Epithelial Cells

Chronic inflammation and elevated cytokine levels are closely associated with the progression of chronic kidney disease (CKD), which is responsible for the manifestation of numerous complications and mortality. In addition to conventional CKD therapies, the possibility of using natural compounds wit...

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Bibliographic Details
Published in:Biomedicines 2023-12, Vol.11 (12), p.3322
Main Authors: Müller, Aline, Lozoya, Maria, Chen, Xiaoying, Weissig, Volkmar, Nourbakhsh, Mahtab
Format: Article
Language:English
Subjects:
1-Phosphatidylinositol 3-kinase
Antibodies
CD27 antigen
Cell adhesion
Cell culture
Chronic kidney failure
Cytokines
Development and progression
Dietary supplements
Epithelial cells
Farnesol
Fluorescent indicators
Gene expression
Genes
Genetic transcription
Health aspects
human
Hydrophobicity
IL-1β
Inflammation
Invoices
Kidney diseases
Kinases
Lipids
Monocyte chemoattractant protein 1
nanoliposomes
Phosphorylation
Protein arrays
Proteins
renal proximal tubule epithelial cells
RNA
Scientific equipment and supplies industry
Signal transduction
Sport-utility vehicles
TNF-α
Transcription activation
Tumor necrosis factor-α
Vesicles
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Summary:Chronic inflammation and elevated cytokine levels are closely associated with the progression of chronic kidney disease (CKD), which is responsible for the manifestation of numerous complications and mortality. In addition to conventional CKD therapies, the possibility of using natural compounds with anti-inflammatory potential has attracted widespread attention in scientific research. This study aimed to study the potential anti-inflammatory effects of a natural oil compound, farnesol, in primary human renal proximal tubule epithelial cell (RPTEC) culture. Farnesol was encapsulated in lipid-based small unilamellar vesicles (SUVs) to overcome its insolubility in cell culture medium. The cell attachment of empty vesicles (SUVs) and farnesol-loaded vesicles (farnesol-SUVs) was examined using BODIPY, a fluorescent dye with hydrophobic properties. Next, we used multiple protein, RNA, and protein phosphorylation arrays to investigate the impact of farnesol on inflammatory signaling in RPTECs. The results indicated that farnesol inhibits TNF-α/IL-1β-induced phosphorylation of the PI3 kinase p85 subunit and subsequent transcriptional activation of the inflammatory genes TNFRSF9, CD27, TNFRSF8, DR6, FAS, IL-7, and CCL2. Therefore, farnesol may be a promising natural compound for treating CKD.
ISSN:2227-9059
2227-9059
DOI:10.3390/biomedicines11123322