Effects of Extract of Arrabidaea chica Verlot on an Experimental Model of Osteoarthritis

• Published in: International journal of molecular sciences 2019-09, Vol.20 (19), p.4717
• Main Authors: Vasconcelos, Cleydlenne Costa, Lopes, Alberto Jorge Oliveira, Sousa, Emerson Lucas Frazão, Camelo, Darleno Sousa, Lima, Fernando César Vilhena Moreira, Rocha, Cláudia Quintino da, Silva, Gyl Eanes Barros, Garcia, João Batista Santos, Cartágenes, Maria do Socorro de Sousa
• Format: Article
• Language: English
• Subjects: Animals; antinociception; Arrabidaea chica; Arthritis; Bignoniaceae - chemistry; bioinformatics; Biomedical materials; Cartilage; Chemical composition; Cyclooxygenase 2 - metabolism; Cyclooxygenase 2 Inhibitors - chemistry; Cyclooxygenase 2 Inhibitors - pharmacology; Cyclooxygenase-2; Disease Models, Animal; Drug dosages; Enzymes; Flavonoids; Flavonoids - chemistry; Flavonoids - pharmacology; High-performance liquid chromatography; Hyperalgesia; Hyperalgesia - chemically induced; Hyperalgesia - diagnostic imaging; Hyperalgesia - drug therapy; In vivo methods and tests; inflammation; Iodoacetic Acid - toxicity; Knee; Metabolites; Molecular docking; Motor activity; Motor Activity - drug effects; Organ Specificity - drug effects; Osteoarthritis; Osteoarthritis - chemically induced; Osteoarthritis - diagnostic imaging; Osteoarthritis - drug therapy; Pain; Pain perception; pariri; phytotherapy; Plant Extracts - chemistry; Plant Extracts - pharmacology; plants; Rats; Rats, Wistar; Spleen; Tumor necrosis factor-TNF
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms20194717

The aim of this study was to analyze the analgesic potential of extract (EHA) as an alternative to osteoarthritis (OA) treatment. Thus, the extract was initially evaluated by the cyclooxygenase inhibition test. The analgesic effect of the extract, in vivo, was also verified in a model of OA induced by sodium monoiodoacetate (2 mg). EHA was administered to rats at doses of 50, 150, and 450 mg/kg between 3 and 25 days after OA induction. The animals were clinically evaluated every 7 days, euthanized at 29 days, and the liver, spleen, kidney and knee collected for histopathological analysis. The chemical composition of EHA was identified by HPLC-MS and the identified compounds submitted to molecular docking study. The results showed that the extract promoted cyclooxygenase inhibition and produced significant improvements in disability, motor activity, hyperalgesia, and OA-induced allodynia parameters, in addition to improvements in the radiological condition of the knees (but not observed in the histopathological study). Chemically the extract is rich in flavonoids. Among them, we evidence that amentoflavone showed very favorable interactions with the enzyme COX-2 in the in silico analysis. Thus, it is concluded that has important analgesic properties for the treatment of OA.