Analysis of the impact of three phthalates on the freshwater gastropod Physella acuta at the transcriptional level

• Published in: Scientific reports 2021-06, Vol.11 (1), p.11411-11411, Article 11411
• Main Authors: Prieto-Amador, Marina, Caballero, Patricia, Martínez-Guitarte, José-Luis
• Format: Article
• Language: English
• Subjects: 704/158/2459; 704/172/4081; Apoptosis; Butyl benzyl phthalate; Cellular stress response; Cytochrome; Detoxification; Diethyl phthalate; DNA methylation; DNA repair; Energy reserves; Environmental impact; Gene expression; Heat shock proteins; Humanities and Social Sciences; Hypoxia; Invertebrates; Kinases; Lipids; Metabolism; Mollusks; multidisciplinary; Oxidative stress; Phthalate esters; Phthalates; Physella acuta; Plastic debris; Plastic pollution; Plastics; Polyvinyl chloride; Science; Science (multidisciplinary); Transcription
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-021-90934-9

Plastic pollution is one of the leading environmental problems. Phthalates are widely used plastic additives released into the environment. Although the effects of phthalates on vertebrates have been extensively studied, there is a knowledge gap regarding their effects on invertebrates. This work analyzes the impact of three phthalates, diethyl phthalate (DEP), benzyl butyl phthalate (BBP), and bis-(2-ethylhexyl) phthalate (DEHP), on the gastropod Physella acuta at the molecular level to establish the putative pathways involved in its response to them. By real-time PCR, we obtained the expression profile of 30 genes in animals exposed for 1 week to 0.1, 10, and 1000 μg/L of each phthalate. The genes cover DNA repair, detoxification, apoptosis, oxidative and stress responses, immunity, energy reserves, and lipid transport. The results show that while DEP and DEHP did not alter the mRNA levels, BBP modulated almost all the analyzed genes. It can be concluded that the impact of BBP is extensive at the molecular level. However, it cannot be dismissed that the increase in transcriptional activity is a general response due to this compound’s well-known role as an endocrine disruptor. Additional research is needed to elucidate the differences observed in the impact of these compounds on the gastropod P . acuta .