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Prevalence and Molecular Characterization of Extended-Spectrum β-Lactamases and AmpC β-lactamase-Producing Enterobacteriaceae among Human, Cattle, and Poultry

Extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae are a universal public health alarm frequently identified among humans, animals, and poultry. Livestock and poultry production are a possible source of multidrug-resistant microorganisms, including ESBL-producing Enterobacteriaceae...

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Published in:Pathogens (Basel) 2022-07, Vol.11 (8), p.852
Main Authors: Nossair, Mohamed A., Abd El Baqy, Fatma A., Rizk, Mohammad S. Y., Elaadli, Haitham, Mansour, Alaa M., Abd El-Aziz, Ayman H., Alkhedaide, Adil, Soliman, Mohamed Mohamed, Ramadan, Hazem, Shukry, Mustafa, Shaaban, Sabah I.
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description Extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae are a universal public health alarm frequently identified among humans, animals, and poultry. Livestock and poultry production are a possible source of multidrug-resistant microorganisms, including ESBL-producing Enterobacteriaceae, which confer antimicrobial resistance to different β-lactam antimicrobial agents. From January to May 2020, a cross-sectional study was carried out in three dairy cattle farms and four poultry farms in different districts of northern Egypt to assess the prevalence of ESBLs, AmpC beta-lactamase-producing E. coli and Klebsiella in livestock, poultry, and human contacts, and to investigate the genetic relatedness of the recovered isolates. In total, 140 samples were collected, including human fecal samples (n = 20) of workers with intimate livestock contact, cattle rectal swabs (n = 34), milk (n = 14), milking machine swabs (n = 8), rations (n = 2), and water (n = 2) from different cattle farms, as well as cloacal swabs (n = 45), rations (n = 5), water (n = 5) and litter (n = 5) from poultry farms. The specimens were investigated for ESBL-producing E. coli and Klebsiella using HiCrome ESBL media agar. The agar disk diffusion method characterized the isolated strains for their phenotypic antimicrobial susceptibility. The prevalence of ESBL-producing Enterobacteriaceae was 30.0%, 20.0%, and 25.0% in humans, cattle, and poultry, respectively. Further genotypic characterization was performed using conventional and multiplex PCR assays for the molecular identification of ESBL and AmpC genes. The majority of the ESBL-producing Enterobacteriaceae showed a multi-drug resistant phenotype. Additionally, blaSHV was the predominant ESBL genotype (n = 31; 93.94%), and was mainly identified in humans (n = 6), cattle (n = 11), and poultry (14); its existence in various reservoirs is a concern, and highlights the necessity of the development of definite control strategies to limit the abuse of antimicrobial agents.
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ispartof Pathogens (Basel), 2022-07, Vol.11 (8), p.852
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source Publicly Available Content Database; PubMed Central
subjects Amides
AmpC
Animals
Antibiotics
Antimicrobial agents
Antimicrobial resistance
Bacteria
Cattle
Dairy cattle
Dairy farms
Drug resistance
E coli
Egypt
Enterobacteriaceae
ESBL
Farms
Farmworkers
Feces
Food
Genes
Genotypes
Health care
Klebsiella
Livestock
Livestock farming
Microorganisms
Milking
Milking machines
Multidrug resistance
Pathogens
Phenotypes
Plasmids
Poultry
Poultry farming
Poultry production
Public health
Rations
Veterinary medicine
β Lactamase
β-Lactam antibiotics
title Prevalence and Molecular Characterization of Extended-Spectrum β-Lactamases and AmpC β-lactamase-Producing Enterobacteriaceae among Human, Cattle, and Poultry
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-01T08%3A19%3A08IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_doaj_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Prevalence%20and%20Molecular%20Characterization%20of%20Extended-Spectrum%20%CE%B2-Lactamases%20and%20AmpC%20%CE%B2-lactamase-Producing%20Enterobacteriaceae%20among%20Human,%20Cattle,%20and%20Poultry&rft.jtitle=Pathogens%20(Basel)&rft.au=Nossair,%20Mohamed%20A.&rft.date=2022-07-28&rft.volume=11&rft.issue=8&rft.spage=852&rft.pages=852-&rft.issn=2076-0817&rft.eissn=2076-0817&rft_id=info:doi/10.3390/pathogens11080852&rft_dat=%3Cproquest_doaj_%3E2707600492%3C/proquest_doaj_%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c470t-d424b294373e782de0ec4c360ec917cdb3c01ad54d9557d16d34038c78ee2dad3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=2706269831&rft_id=info:pmid/36014973&rfr_iscdi=true