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Prevalence and Molecular Characterization of Extended-Spectrum β-Lactamases and AmpC β-lactamase-Producing Enterobacteriaceae among Human, Cattle, and Poultry
Extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae are a universal public health alarm frequently identified among humans, animals, and poultry. Livestock and poultry production are a possible source of multidrug-resistant microorganisms, including ESBL-producing Enterobacteriaceae...
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Published in: | Pathogens (Basel) 2022-07, Vol.11 (8), p.852 |
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description | Extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae are a universal public health alarm frequently identified among humans, animals, and poultry. Livestock and poultry production are a possible source of multidrug-resistant microorganisms, including ESBL-producing Enterobacteriaceae, which confer antimicrobial resistance to different β-lactam antimicrobial agents. From January to May 2020, a cross-sectional study was carried out in three dairy cattle farms and four poultry farms in different districts of northern Egypt to assess the prevalence of ESBLs, AmpC beta-lactamase-producing E. coli and Klebsiella in livestock, poultry, and human contacts, and to investigate the genetic relatedness of the recovered isolates. In total, 140 samples were collected, including human fecal samples (n = 20) of workers with intimate livestock contact, cattle rectal swabs (n = 34), milk (n = 14), milking machine swabs (n = 8), rations (n = 2), and water (n = 2) from different cattle farms, as well as cloacal swabs (n = 45), rations (n = 5), water (n = 5) and litter (n = 5) from poultry farms. The specimens were investigated for ESBL-producing E. coli and Klebsiella using HiCrome ESBL media agar. The agar disk diffusion method characterized the isolated strains for their phenotypic antimicrobial susceptibility. The prevalence of ESBL-producing Enterobacteriaceae was 30.0%, 20.0%, and 25.0% in humans, cattle, and poultry, respectively. Further genotypic characterization was performed using conventional and multiplex PCR assays for the molecular identification of ESBL and AmpC genes. The majority of the ESBL-producing Enterobacteriaceae showed a multi-drug resistant phenotype. Additionally, blaSHV was the predominant ESBL genotype (n = 31; 93.94%), and was mainly identified in humans (n = 6), cattle (n = 11), and poultry (14); its existence in various reservoirs is a concern, and highlights the necessity of the development of definite control strategies to limit the abuse of antimicrobial agents. |
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Y. ; Elaadli, Haitham ; Mansour, Alaa M. ; Abd El-Aziz, Ayman H. ; Alkhedaide, Adil ; Soliman, Mohamed Mohamed ; Ramadan, Hazem ; Shukry, Mustafa ; Shaaban, Sabah I.</creator><creatorcontrib>Nossair, Mohamed A. ; Abd El Baqy, Fatma A. ; Rizk, Mohammad S. Y. ; Elaadli, Haitham ; Mansour, Alaa M. ; Abd El-Aziz, Ayman H. ; Alkhedaide, Adil ; Soliman, Mohamed Mohamed ; Ramadan, Hazem ; Shukry, Mustafa ; Shaaban, Sabah I.</creatorcontrib><description>Extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae are a universal public health alarm frequently identified among humans, animals, and poultry. Livestock and poultry production are a possible source of multidrug-resistant microorganisms, including ESBL-producing Enterobacteriaceae, which confer antimicrobial resistance to different β-lactam antimicrobial agents. From January to May 2020, a cross-sectional study was carried out in three dairy cattle farms and four poultry farms in different districts of northern Egypt to assess the prevalence of ESBLs, AmpC beta-lactamase-producing E. coli and Klebsiella in livestock, poultry, and human contacts, and to investigate the genetic relatedness of the recovered isolates. In total, 140 samples were collected, including human fecal samples (n = 20) of workers with intimate livestock contact, cattle rectal swabs (n = 34), milk (n = 14), milking machine swabs (n = 8), rations (n = 2), and water (n = 2) from different cattle farms, as well as cloacal swabs (n = 45), rations (n = 5), water (n = 5) and litter (n = 5) from poultry farms. The specimens were investigated for ESBL-producing E. coli and Klebsiella using HiCrome ESBL media agar. The agar disk diffusion method characterized the isolated strains for their phenotypic antimicrobial susceptibility. The prevalence of ESBL-producing Enterobacteriaceae was 30.0%, 20.0%, and 25.0% in humans, cattle, and poultry, respectively. Further genotypic characterization was performed using conventional and multiplex PCR assays for the molecular identification of ESBL and AmpC genes. The majority of the ESBL-producing Enterobacteriaceae showed a multi-drug resistant phenotype. Additionally, blaSHV was the predominant ESBL genotype (n = 31; 93.94%), and was mainly identified in humans (n = 6), cattle (n = 11), and poultry (14); its existence in various reservoirs is a concern, and highlights the necessity of the development of definite control strategies to limit the abuse of antimicrobial agents.</description><identifier>ISSN: 2076-0817</identifier><identifier>EISSN: 2076-0817</identifier><identifier>DOI: 10.3390/pathogens11080852</identifier><identifier>PMID: 36014973</identifier><language>eng</language><publisher>Basel: MDPI AG</publisher><subject>Amides ; AmpC ; Animals ; Antibiotics ; Antimicrobial agents ; Antimicrobial resistance ; Bacteria ; Cattle ; Dairy cattle ; Dairy farms ; Drug resistance ; E coli ; Egypt ; Enterobacteriaceae ; ESBL ; Farms ; Farmworkers ; Feces ; Food ; Genes ; Genotypes ; Health care ; Klebsiella ; Livestock ; Livestock farming ; Microorganisms ; Milking ; Milking machines ; Multidrug resistance ; Pathogens ; Phenotypes ; Plasmids ; Poultry ; Poultry farming ; Poultry production ; Public health ; Rations ; Veterinary medicine ; β Lactamase ; β-Lactam antibiotics</subject><ispartof>Pathogens (Basel), 2022-07, Vol.11 (8), p.852</ispartof><rights>2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). 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Y.</creatorcontrib><creatorcontrib>Elaadli, Haitham</creatorcontrib><creatorcontrib>Mansour, Alaa M.</creatorcontrib><creatorcontrib>Abd El-Aziz, Ayman H.</creatorcontrib><creatorcontrib>Alkhedaide, Adil</creatorcontrib><creatorcontrib>Soliman, Mohamed Mohamed</creatorcontrib><creatorcontrib>Ramadan, Hazem</creatorcontrib><creatorcontrib>Shukry, Mustafa</creatorcontrib><creatorcontrib>Shaaban, Sabah I.</creatorcontrib><title>Prevalence and Molecular Characterization of Extended-Spectrum β-Lactamases and AmpC β-lactamase-Producing Enterobacteriaceae among Human, Cattle, and Poultry</title><title>Pathogens (Basel)</title><description>Extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae are a universal public health alarm frequently identified among humans, animals, and poultry. Livestock and poultry production are a possible source of multidrug-resistant microorganisms, including ESBL-producing Enterobacteriaceae, which confer antimicrobial resistance to different β-lactam antimicrobial agents. From January to May 2020, a cross-sectional study was carried out in three dairy cattle farms and four poultry farms in different districts of northern Egypt to assess the prevalence of ESBLs, AmpC beta-lactamase-producing E. coli and Klebsiella in livestock, poultry, and human contacts, and to investigate the genetic relatedness of the recovered isolates. In total, 140 samples were collected, including human fecal samples (n = 20) of workers with intimate livestock contact, cattle rectal swabs (n = 34), milk (n = 14), milking machine swabs (n = 8), rations (n = 2), and water (n = 2) from different cattle farms, as well as cloacal swabs (n = 45), rations (n = 5), water (n = 5) and litter (n = 5) from poultry farms. The specimens were investigated for ESBL-producing E. coli and Klebsiella using HiCrome ESBL media agar. The agar disk diffusion method characterized the isolated strains for their phenotypic antimicrobial susceptibility. The prevalence of ESBL-producing Enterobacteriaceae was 30.0%, 20.0%, and 25.0% in humans, cattle, and poultry, respectively. Further genotypic characterization was performed using conventional and multiplex PCR assays for the molecular identification of ESBL and AmpC genes. The majority of the ESBL-producing Enterobacteriaceae showed a multi-drug resistant phenotype. 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Y.</au><au>Elaadli, Haitham</au><au>Mansour, Alaa M.</au><au>Abd El-Aziz, Ayman H.</au><au>Alkhedaide, Adil</au><au>Soliman, Mohamed Mohamed</au><au>Ramadan, Hazem</au><au>Shukry, Mustafa</au><au>Shaaban, Sabah I.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Prevalence and Molecular Characterization of Extended-Spectrum β-Lactamases and AmpC β-lactamase-Producing Enterobacteriaceae among Human, Cattle, and Poultry</atitle><jtitle>Pathogens (Basel)</jtitle><date>2022-07-28</date><risdate>2022</risdate><volume>11</volume><issue>8</issue><spage>852</spage><pages>852-</pages><issn>2076-0817</issn><eissn>2076-0817</eissn><abstract>Extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae are a universal public health alarm frequently identified among humans, animals, and poultry. Livestock and poultry production are a possible source of multidrug-resistant microorganisms, including ESBL-producing Enterobacteriaceae, which confer antimicrobial resistance to different β-lactam antimicrobial agents. From January to May 2020, a cross-sectional study was carried out in three dairy cattle farms and four poultry farms in different districts of northern Egypt to assess the prevalence of ESBLs, AmpC beta-lactamase-producing E. coli and Klebsiella in livestock, poultry, and human contacts, and to investigate the genetic relatedness of the recovered isolates. In total, 140 samples were collected, including human fecal samples (n = 20) of workers with intimate livestock contact, cattle rectal swabs (n = 34), milk (n = 14), milking machine swabs (n = 8), rations (n = 2), and water (n = 2) from different cattle farms, as well as cloacal swabs (n = 45), rations (n = 5), water (n = 5) and litter (n = 5) from poultry farms. The specimens were investigated for ESBL-producing E. coli and Klebsiella using HiCrome ESBL media agar. The agar disk diffusion method characterized the isolated strains for their phenotypic antimicrobial susceptibility. The prevalence of ESBL-producing Enterobacteriaceae was 30.0%, 20.0%, and 25.0% in humans, cattle, and poultry, respectively. Further genotypic characterization was performed using conventional and multiplex PCR assays for the molecular identification of ESBL and AmpC genes. The majority of the ESBL-producing Enterobacteriaceae showed a multi-drug resistant phenotype. Additionally, blaSHV was the predominant ESBL genotype (n = 31; 93.94%), and was mainly identified in humans (n = 6), cattle (n = 11), and poultry (14); its existence in various reservoirs is a concern, and highlights the necessity of the development of definite control strategies to limit the abuse of antimicrobial agents.</abstract><cop>Basel</cop><pub>MDPI AG</pub><pmid>36014973</pmid><doi>10.3390/pathogens11080852</doi><orcidid>https://orcid.org/0000-0002-6687-3758</orcidid><orcidid>https://orcid.org/0000-0001-7208-7123</orcidid><orcidid>https://orcid.org/0000-0003-2722-2466</orcidid><orcidid>https://orcid.org/0000-0002-2589-6988</orcidid><orcidid>https://orcid.org/0000-0002-8306-0011</orcidid><oa>free_for_read</oa></addata></record> |
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subjects | Amides AmpC Animals Antibiotics Antimicrobial agents Antimicrobial resistance Bacteria Cattle Dairy cattle Dairy farms Drug resistance E coli Egypt Enterobacteriaceae ESBL Farms Farmworkers Feces Food Genes Genotypes Health care Klebsiella Livestock Livestock farming Microorganisms Milking Milking machines Multidrug resistance Pathogens Phenotypes Plasmids Poultry Poultry farming Poultry production Public health Rations Veterinary medicine β Lactamase β-Lactam antibiotics |
title | Prevalence and Molecular Characterization of Extended-Spectrum β-Lactamases and AmpC β-lactamase-Producing Enterobacteriaceae among Human, Cattle, and Poultry |
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