Modulation of drug resistance and biofilm formation of Staphylococcus aureus isolated from the oral cavity of Tunisian children

This study aims to investigate the antimicrobial and the anti-biofilm activities of Lactobacillus plantarum extract (LPE) against a panel of oral Staphylococcus aureus (n=9) and S. aureus ATCC 25923. The in vitro ability of LPE to modulate bacterial resistance to tetracycline, benzalchonium chloride...

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Bibliographic Details
Published in:The Brazilian journal of infectious diseases 2017-01, Vol.21 (1), p.27-34
Main Authors: Zmantar, Tarek, Ben Slama, Rihab, Fdhila, Kais, Kouidhi, Bochra, Bakhrouf, Amina, Chaieb, Kamel
Format: Article
Language:English
Subjects:
Anti-Bacterial Agents - pharmacology
Anti-infective agents
Anti-Infective Agents, Local - pharmacology
Antibacterial
Antibiotics
Benzalkonium Compounds - pharmacology
Biofilm
Biofilms - drug effects
Biofilms - growth & development
Care and treatment
Child
Chlorhexidine - pharmacology
Dosage and administration
Drug resistance in microorganisms
Drug Resistance, Bacterial - drug effects
Humans
INFECTIOUS DISEASES
Lactobacillus
Lactobacillus plantarum
Lactobacillus plantarum - chemistry
Microbial Sensitivity Tests
Microscopy, Atomic Force - methods
Mouth - microbiology
Original
Polymerase Chain Reaction
Reference Values
Reproducibility of Results
Staphylococcus aureus
Staphylococcus aureus - drug effects
Staphylococcus aureus - isolation & purification
Staphylococcus aureus infections
Statistics, Nonparametric
Synergetic
Tetracycline - pharmacology
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Summary:This study aims to investigate the antimicrobial and the anti-biofilm activities of Lactobacillus plantarum extract (LPE) against a panel of oral Staphylococcus aureus (n=9) and S. aureus ATCC 25923. The in vitro ability of LPE to modulate bacterial resistance to tetracycline, benzalchonium chloride, and chlorhexidine were tested also. The minimum inhibitory concentrations (MICs) and the minimal bactericidal concentrations of Lactobacillus plantarum extract, tetracycline, benzalchonium chloride and clohrhexidine were determined in absence and in presence of a sub-MIC doses of LPE (1/2 MIC). In addition, the LPE potential to inhibit biofilm formation was assessed by microtiter plate and atomic force microscopy assays. Statistical analysis was performed on SPSS v. 17.0 software using Friedman test and Wilcoxon signed ranks test. These tests were used to assess inter-group difference (p
ISSN:1413-8670
1678-4391
1678-4391
DOI:10.1016/j.bjid.2016.10.009