A Customized Two Photon Fluorescence Imaging Probe Based on 2D scanning MEMS Mirror Including Electrothermal Two-Level-Ladder Dual S-Shaped Actuators

We report the design and characterization of a two-photon fluorescence imaging miniature probe. This customized two-axis scanning probe is dedicated for intraoperative two-photon fluorescence imaging endomicroscopic use and is based on a micro-electro-mechanical system (MEMS) mirror with a high refl...

Full description

Saved in:
Bibliographic Details
Published in:Micromachines (Basel) 2020-07, Vol.11 (7), p.704
Main Authors: Mehidine, Hussein, Li, Min, Lendresse, Jean-Francois, Bouvet, Francoise, Xie, Huikai, Abi Haidar, Darine
Format: Article
Language:English
Subjects:
2D scanning probe
Actuators
Apertures
Biopsy
Brain cancer
Design
Diameters
Dwell time
electrothermal actuators
Endoscopy
Fluorescence
Imaging
Lasers
Mechanical systems
micro-electro-mechanical system (MEMS) mirror
Microelectromechanical systems
Microscopy
Optics
Photons
Physics
Pulse duration
Raster scanning
Scanners
Surgeons
Surgery
Tumors
two-photon imaging
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:We report the design and characterization of a two-photon fluorescence imaging miniature probe. This customized two-axis scanning probe is dedicated for intraoperative two-photon fluorescence imaging endomicroscopic use and is based on a micro-electro-mechanical system (MEMS) mirror with a high reflectivity plate and two-level-ladder double S-shaped electrothermal bimorph actuators. The fully assembled probe has a total outer diameter of 4 mm including all elements. With a two-lens configuration and a small aperture MEMS mirror, this probe can generate a large optical scan angle of 24° with 4 V drive voltage and can achieve a 450 µm FOV with a 2-fps frame rate. A uniform Pixel Dwell Time and a stable scanning speed along a raster pattern were demonstrated while a 57-fs pulse duration of the excitation beam was measured at the exit of the probe head. This miniature imaging probe will be coupled to a two-photon fluorescence endomicroscope oriented towards clinical use.
ISSN:2072-666X
2072-666X
DOI:10.3390/mi11070704