Development of Green HPTLC method for simultaneous determination of a promising combination Tamsulosin and Mirabegron: stability-indicating assay was examined

Recently, mirabegron has been added to tamsulosin to treat overactive bladder in men with benign prostatic hypertrophy. A Rapid, selective, sensitive, and green high-performance thin-layer chromatography (HPTLC) approach was developed for the simultaneous determination of tamsulosin (TAM) and mirabe...

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Published in:BMC chemistry 2023-09, Vol.17 (1), p.130-130, Article 130
Main Authors: Abou El-Alamin, Maha M., Toubar, Safaa S., Mohamed, Dina A., Helmy, Marwa I.
Format: Article
Language:English
Subjects:
Acetates
Ammonia
Analytical chemistry
Chemistry
Chemistry and Materials Science
Chemistry/Food Science
Chromatography
Esters
Ethyl acetate
Green assessment
HPTLC
Ions
Mathematical analysis
Methanol
Methods
Mirabegron
Silica gel
Stability analysis
Stability study
Statistical analysis
Sustainable development
Tamsulosin
Tamsulosin hydrochloride
Thin layer chromatography
Ultraviolet radiation
Urinary incontinence
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Summary:Recently, mirabegron has been added to tamsulosin to treat overactive bladder in men with benign prostatic hypertrophy. A Rapid, selective, sensitive, and green high-performance thin-layer chromatography (HPTLC) approach was developed for the simultaneous determination of tamsulosin (TAM) and mirabegron (MIR) in pure and laboratory-prepared mixture. Complete separation was obtained on silica gel F 254 using the solvent system methanol-ethyl acetate-ammonia (3:7:0.1, v/v). Short-wave ultraviolet light at 270 nm was used to view the chromatographic bands. For MIR and TAM, the suggested technique revealed compact spots with retention factor R f values of 0.42 and 0.63, respectively. Within concentration ranges of 0.15–7.5 µg/band and 0.05–2.5 µg/band, good linearity was observed, with mean percentage recoveries of 100.04 ± 0.56 and 99.98% ± 0.95 for MIR and TAM, respectively. Green assessment of the developed HPTLC technique was estimated using different green analytical chemistry metrics such as Analytical eco-scale Analytical GREEness (AGREE), and Green Analytical Procedure Index (GAPI) metrics. The proposed method was effectively used as a stability-indicating assay to assess the presence of MIR and TAM in the pharmaceutical dosage form in the presence of their degradation product. The statistical analysis showed high precision and accuracy.
ISSN:2661-801X
2661-801X
DOI:10.1186/s13065-023-01043-9