Expression of serotonergic (5HT) receptors in patients with chronic rhinosinusitis with nasal polyposis and in normal nasal mucosa

• Published in: The Egyptian journal of otolaryngology 2024-12, Vol.40 (1), p.44-9, Article 44
• Main Authors: Keshk, Ahmed Osama Tolba, Sabry, Sabry Magdy, Fawaz, Samia Ahmed, Salman, Manal Ibrahim, El-Megeed, Heba Mahmoud Abd
• Format: Article
• Language: English
• Subjects: Allergic reaction; Allergic rhinitis; Allergy; Antihistamines; Biomarkers; Blood vessels; Care and treatment; Chronic rhinosinusitis; Cytokines; Endoscopy; Histamine; Hypotheses; Immunohistochemistry; Medical imaging; Medical research; Medicine; Medicine & Public Health; Medicine, Experimental; Nasal polypi; Nose; Original Article; Otolaryngology; Polyps; Serotonin; Serotonin receptors; Stains & staining; Steroids; Surgery
• ISSN: 2090-8539; 1012-5574; 2090-8539
• DOI: 10.1186/s43163-024-00601-9

Expression of serotonergic (5HT) receptors in patients with chronic rhinosinusitis with nasal polyposis and in normal nasal mucosa: a case-control study. Background  Chronic rhinosinusitis with nasal polyposis is one of the challenging conditions regarding treatment and management. Many recent studies introduced new modalities for treatment like targeted immunomodulating drugs rather than antihistamines, local and systemic steroids, and endoscopic sinus surgery. Understanding the pathology behind the disease by studying its immunologic pathways and substances that take part in the inflammatory process can help in the introduction of new treatment options that can improve outcomes and decrease the costs of treatment. Objective  The aim of our study is to demonstrate the presence of 5-HT receptors in nasal polyps and inferior turbinate mucosa in patients with chronic rhinosinusitis with nasal polyposis and then compare this expression of 5-HT receptors with inferior turbinate mucosa of the normal control group. Methods  A case-control study was conducted on 80 patients. Specimens from nasal polypi and turbinate mucosa of patients with chronic rhinosinusitis (cases) and specimens from normal turbinate mucosa (controls) were stained with serotonin receptor Immunohistochemical staining by automated immunostainer in the pathology department laboratory in Ain Shams University Hospital with ratio 1:200–400 as recommended by the manufacturing company. Then, compared as regards stain distribution and intensity of mucosal lining, glands, and blood vessels. Results  There was a significant difference between polyp cases and controls as regards lining epithelium and mucosal gland stain distribution and stain intensity. Also significant difference between the polyp cases and controls as regards blood vessel strain distribution. However, no significant difference was found as regards blood vessel stain intensity. There was a significant difference between turbinate cases and controls as regards lining epithelium stain distribution, and stain intensity. There was no significant difference between turbinate cases and controls as regards gland stain distribution. However, a significant difference was found between turbinate and controls as regards gland stain intensity. There was a significant difference between turbinate cases and controls as regards blood vessel stain distribution; however, no significant difference was present as regards stain intensity. There was no sig