Ultra-Fast Impedimetric Immunoassay for Detection of Streptococcus agalactiae Using Carbon Electrode with Nanodiamonds Film

This publication presents the results of work on the development of a quick and cheap electrochemical immunosensor for the diagnosis of infections with the pathogen . The research was carried out on the basis of the modification of the well-known glassy carbon (GC) electrodes. The surface of the GC...

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Bibliographic Details
Published in:Micromachines (Basel) 2023-05, Vol.14 (5), p.1076
Main Authors: Bigus, Daniel, Lewandowska, Wioleta, Bięga, Ewelina, Grela, Anna, Siedlar, Aleksandra, Sosnowska, Marta, Fabisiak, Magdalena, Łęga, Tomasz, Dashkievich, Yanina, Nowacka-Dośpiał, Joanna, Palka, Katarzyna, Żołędowska, Sabina, Nidzworski, Dawid
Format: Article
Language:English
Subjects:
Antibiotics
Antibodies
Antigens
Bacteria
Carbon
Diamonds
Disease prevention
E coli
Electrochemical impedance spectroscopy
Electrodes
Ethyldimethylaminopropyl carbodiimide
Glassy carbon
glassy carbon electrode
immobilization
Immunoassay
immunosensor
Immunosensors
Laboratories
Meningitis
Microorganisms
nanodiamonds
Nanostructure
Newborn babies
Pathogens
Potassium
potato starch
Pregnancy
Proteins
Sensors
Sepsis
streptococcus agalactiae
Streptococcus infections
Womens health
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Summary:This publication presents the results of work on the development of a quick and cheap electrochemical immunosensor for the diagnosis of infections with the pathogen . The research was carried out on the basis of the modification of the well-known glassy carbon (GC) electrodes. The surface of the GC (glassy carbon) electrode was covered with a film made of nanodiamonds, which increased the number of sites for the attachment of anti- antibodies. The GC surface was activated with EDC/NHS (1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide/N-Hydroxysuccinimide). Determination of electrode characteristics after each modification step, performed using cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS).
ISSN:2072-666X
2072-666X
DOI:10.3390/mi14051076