A high-resolution phase-contrast microscopy system for label-free imaging in living cells

Cell biologists have long sought the ability to observe intracellular structures in living cells without labels. This study presents procedures to adjust a commercially available apodized phase-contrast (APC) microscopy system for better visualizing the dynamic behaviors of various subcellular organ...

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Bibliographic Details
Published in:Cell Structure and Function 2024, Vol.49(1), pp.21-29
Main Authors: Shimasaki, Kentaro, Okemoto-Nakamura, Yuko, Saito, Kyoko, Fukasawa, Masayoshi, Katoh, Kaoru, Hanada, Kentaro
Format: Article
Language:English
Subjects:
Animals
Antibodies
apodized phase contrast
Heat
HeLa Cells
Humans
label-free imaging
Microscopy
Microscopy, Phase-Contrast - methods
organelle dynamics
Organelles
Organelles - ultrastructure
Penicillin
Phenols
Technical Note
virus infections
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Summary:Cell biologists have long sought the ability to observe intracellular structures in living cells without labels. This study presents procedures to adjust a commercially available apodized phase-contrast (APC) microscopy system for better visualizing the dynamic behaviors of various subcellular organelles in living cells. By harnessing the versatility of this technique to capture sequential images, we could observe morphological changes in cellular geometry after virus infection in real time without probes or invasive staining. The tune-up APC microscopy system is a highly efficient platform for simultaneously observing the dynamic behaviors of diverse subcellular structures with exceptional resolution.
ISSN:0386-7196
1347-3700
1347-3700
DOI:10.1247/csf.24018