Antioxidant and Cytoprotective Effects of (-)-Epigallocatechin-3-(3″- O -methyl) Gallate

• Published in: International journal of molecular sciences 2019-08, Vol.20 (16), p.3993
• Main Authors: Kim, Eunji, Han, Sang Yun, Hwang, Kyeonghwan, Kim, Donghyun, Kim, Eun-Mi, Hossain, Mohammad Amjad, Kim, Jong-Hoon, Cho, Jae Youl
• Format: Article
• Language: English
• Subjects: (−)-Epigallocatechin-3-(3″-O-methyl) gallate (3″Me-EGCG); Aging; AKT1; AKT1 protein; AKT2 protein; antioxidant; Antioxidants; Antioxidants - chemistry; Antioxidants - pharmacology; Apoptosis; Catechin - analogs & derivatives; Catechin - chemistry; Catechin - pharmacology; Cell growth; Cell Line; Cell proliferation; Cell Survival - drug effects; Cell Survival - radiation effects; Cultivars; cytoprotection; Cytoprotection - drug effects; Cytoprotection - radiation effects; Deoxyribonucleic acid; DNA; DNA-dependent protein kinase; Epigallocatechin gallate; Gallic Acid - analogs & derivatives; Gallic Acid - chemistry; Gallic Acid - pharmacology; Humans; Inflammatory diseases; Keratinocytes - cytology; Keratinocytes - drug effects; Keratinocytes - metabolism; Keratinocytes - radiation effects; Kinases; Oxidative stress; Plant extracts; Protein kinase C; Radiation-Protective Agents - chemistry; Radiation-Protective Agents - pharmacology; Reactive Oxygen Species - metabolism; Tea; Ultraviolet Rays - adverse effects
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms20163993

Reactive oxygen species (ROS) are generated from diverse cellular processes or external sources such as chemicals, pollutants, or ultraviolet (UV) irradiation. Accumulation of radicals causes cell damage that can result in degenerative diseases. Antioxidants remove radicals by eliminating unpaired electrons from other molecules. In skin health, antioxidants are essential to protect cells from the environment and prevent skin aging. (-)-Epigallocatechin-3-(3″- -methyl) gallate (3″Me-EGCG) has been found in limited oolong teas or green teas with distinctive methylated form, but its precise activities have not been fully elucidated. In this study, we examined the antioxidant roles of 3″Me-EGCG in keratinocytes (HaCaT cells). 3″Me-EGCG showed scavenging effects in cell and cell-free systems. Under H O exposure, 3″Me-EGCG recovered cell viability and increased the expression of heme oxygenase 1 (HO-1). Under ultraviolet B (UVB) and sodium nitroprusside (SNP) exposure, 3″Me-EGCG protected keratinocytes and regulated the survival protein AKT1. By regulating the AKT1/NF-κB pathway, 3″Me-EGCG augmented cell survival and proliferation in HaCaT cells. These results indicate that 3″Me-EGCG exhibits antioxidant properties, resulting in cytoprotection against various external stimuli. In conclusion, our findings suggest that 3″Me-EGCG can be used as an ingredient of cosmetic products or health supplements.