Phage-mediated resolution of genetic conflict alters the evolutionary trajectory of Pseudomonas aeruginosa lysogens

• Published in: mSystems 2024-09, Vol.9 (9), p.e0080124
• Main Authors: Suttenfield, Laura C, Rapti, Zoi, Chandrashekhar, Jayadevi H, Steinlein, Amelia C, Vera, Juan Cristobal, Kim, Ted, Whitaker, Rachel J
• Format: Article
• Language: English
• Subjects: Bacteriophages; Bacteriophages - genetics; Clustered Regularly Interspaced Short Palindromic Repeats - genetics; CRISPR; Editor’s Pick; evolution; Evolution, Molecular; lysogen; Lysogeny - genetics; Prophages - genetics; Pseudomonas aeruginosa - genetics; Pseudomonas aeruginosa - virology; Pseudomonas Phages - genetics; Research Article; spontaneous induction; transposable phages
• ISSN: 2379-5077; 2379-5077
• DOI: 10.1128/msystems.00801-24

The opportunistic human pathogen is naturally infected by a large class of temperate, transposable, Mu-like phages. We examined the genotypic and phenotypic diversity of PA14 lysogen populations as they resolve clustered regularly interspaced short palindromic repeat CRISPR) autoimmunity, mediated by an imperfect CRISPR match to the Mu-like DMS3 prophage. After 12 days of evolution, we measured a decrease in spontaneous induction in both exponential and stationary phase growth. Co-existing variation in spontaneous induction rates in the exponential phase depended on the way the coexisting strains resolved genetic conflict. Multiple mutational modes to resolve genetic conflict between host and phage resulted in coexistence in evolved populations of single lysogens that maintained CRISPR immunity to other phages and polylysogens that lost immunity completely. This work highlights a new dimension of the role of lysogenic phages in the evolution of their hosts.IMPORTANCEThe chronic opportunistic multi-drug-resistant pathogen is persistently infected by temperate phages. We assess the contribution of temperate phage infection to the evolution of the clinically relevant strain UCBPP-PA14. We found that a low level of clustered regularly interspaced short palindromic repeat (CRISPR)-mediated self-targeting resulted in polylysogeny evolution and large genome rearrangements in lysogens; we also found extensive diversification in CRISPR spacers and genes. These genomic modifications resulted in decreased spontaneous induction in both exponential and stationary phase growth, increasing lysogen fitness. This work shows the importance of considering latent phage infection in characterizing the evolution of bacterial populations.