A method for the permeabilization of live Drosophila melanogaster larvae to small molecules and cryoprotectants

The larvae of is a model organism widely used to study the muscular and nervous systems. larvae are surrounded by a waxy cuticle that prevents permeation by most substances. Here we develop a method to remove this layer, rendering the larvae permeable to small molecules without causing death, allowi...

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Bibliographic Details
Published in:Fly (Austin, Tex.) Tex.), 2020-10, Vol.14 (1-4), p.29-33
Main Authors: Murray, Alex, Palmer, Daniel, Bennett, Daimark, Dwarampudi, Venkata, Pedro de Magalhães, João
Format: Article
Language:English
Subjects:
cryobiology
cryopreservation
drosophila
imaging
larvae
method
Methods and Technical Advances
permeabilization
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Summary:The larvae of is a model organism widely used to study the muscular and nervous systems. larvae are surrounded by a waxy cuticle that prevents permeation by most substances. Here we develop a method to remove this layer, rendering the larvae permeable to small molecules without causing death, allowing the larvae to develop to adulthood and reproduce. Permeability was assessed using fluorescein diacetate dye uptake, and mortality upon exposure to toxic levels of ethylene glycol (EG) and Dimethyl sulfoxide (DMSO). Potential uses for this method include drug delivery, toxicity assays, cryopreservation, staining, and fixation.
ISSN:1933-6934
1933-6942
DOI:10.1080/19336934.2020.1724051