Monitoring the decontamination efficacy of the novel Poseidon-S disinfectant system in dental unit water lines

Abstract Background Contaminated dental unit waterlines (DUWLs) are a known source of specific health care-acquired infections because of the difficulty in keeping them clean during routine dental practice. Recently, an electrolysis apparatus that uses only the chlorine normally present in municipal...

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Bibliographic Details
Published in:Journal of microbiology, immunology and infection immunology and infection, 2017-06, Vol.50 (3), p.270-276
Main Authors: Fujita, Mari, Mashima, Izumi, Nakazawa, Futoshi
Format: Article
Language:English
Subjects:
Bacteria - isolation & purification
Cell Line - drug effects
Cell Line - physiology
Cell Survival - drug effects
Colony Count, Microbial
cytotoxicity
Decontamination - methods
Dental Offices
dental unit water lines (DUWLs)
Disinfectants - pharmacology
Disinfectants - toxicity
Disinfection - methods
disinfection system
electrolyzed water
Humans
Infectious Disease
Medical Education
microbial contamination
Water Microbiology
Water Supply
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Summary:Abstract Background Contaminated dental unit waterlines (DUWLs) are a known source of specific health care-acquired infections because of the difficulty in keeping them clean during routine dental practice. Recently, an electrolysis apparatus that uses only the chlorine normally present in municipal water, the Poseidon-S system, was developed as a novel additive-free disinfectant system to control microbial contamination in DUWLs. Methods The microbiological quality of water samples collected from DUWLs was assessed before and after installation of the Poseidon-S system in terms of the total viable counts (TVCs) of microorganisms. The microbicidal effects of the electrolyzed water against oral organisms and its cytotoxicity against human oral-derived cell lines were also examined. Results Water samples from the DUWLs initially had average microbial TVCs of 103 –106 colony-forming units (CFU)/mL. After installation of the Poseidon-S system, the number of microorganisms in the water samples decreased to less than 1 × 102  CFU/mL. The electrolyzed water also exhibited remarkable microbicidal effects on the microorganisms present in the DUWLs as well as microorganisms commonly isolated from human oral cavities, but showed low cytotoxicity towards human oral-derived cells. Conclusion This study demonstrated that routine use of the Poseidon-S system can effectively maintain low microbial levels in DUWLs.
ISSN:1684-1182
1995-9133
DOI:10.1016/j.jmii.2015.05.006