The Protective Effect of a Functional Food Consisting of Astragalus membranaceus , Trichosanthes kirilowii , and Angelica gigas or Its Active Component Formononetin against Inflammatory Skin Disorders through Suppression of TSLP via MDM2/HIF1α Signaling Pathways

• Published in: Foods 2023-01, Vol.12 (2), p.276
• Main Authors: Han, Na-Ra, Park, Hi-Joon, Ko, Seong-Gyu, Moon, Phil-Dong
• Format: Article
• Language: English
• Subjects: Acetic acid; Angelica gigas; Antibodies; Astragalus membranaceus; Calcimycin; Cancer; Carcinoma; Communication; Cytotoxicity; Disorders; Ear; Edema; Food; Food science; formononetin; functional food; Functional foods & nutraceuticals; Gene expression; Growth factors; human mast cell line-1; Hypoxia; Hypoxia-inducible factor 1a; Hypoxia-inducible factors; Immunosuppression; Inflammation; JRP-SNF102; MDM2 protein; Mixtures; NF-κB protein; Phorbol 12-myristate 13-acetate; Skin diseases; Thymic stromal lymphopoietin; Thymus; Trichosanthes kirilowii; Vascular endothelial growth factor
• ISSN: 2304-8158; 2304-8158
• DOI: 10.3390/foods12020276

An herbal mixture (SH003) of , , and exhibits therapeutic effects on carcinomas and immunosuppression. However, the role of JRP-SNF102, which is an advanced mixture of SH003, in regulating inflammatory responses is unexplored. We aim to substantiate the therapeutic potential of JRP-SNF102 and its active component, formononetin (FMN), as a functional food that moderates inflammatory responses. The inhibitory effects of JRP-SNF102 or FMN on thymic stromal lymphopoietin (TSLP) levels were evaluated in phorbol 12-myristate 13-acetate (PMA) plus A23187-activated human mast cell line-1 (HMC-1) cells and a mouse model of PMA-induced ear edema. The JRP-SNF102 or FMN inhibited the secretion and mRNA expression of TSLP and vascular endothelial growth factor (VEGF) in the activated HMC-1 cells. The expression levels of murine double minute 2 (MDM2), hypoxia-inducible factor 1α (HIF1α), and NF-κB were also suppressed by JRP-SNF102 or FMN in the activated HMC-1 cells. The JRP-SNF102 or FMN inhibited TSLP and VEGF levels, attenuating redness and ear thickness in mice with acute ear edema; JRP-SNF102 or FMN reduced the expression levels of MDM2, HIF1α, and NF-κB in the ear tissues. These findings suggest the potential for JRP-SNF102 as a functional food in the treatment of inflammatory skin disorders through suppression of TSLP and VEGF.