Intraspecific Variability and Distribution Difference within the Ribosomal Introns of the Discrete Plasmodiophora brassicae Group in Japan: A Case Study for Complex Dynamics of Intron Evolution

Analysis of the ribosomal introns of Plasmodiophora brassicae populations infecting the cruciferous weed Cardamine occulta revealed the complex dynamics of size, intraspecific variability, and distribution. The results showed that P. brassicae populations from the weed have lost multiple introns in...

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Bibliographic Details
Published in:Agronomy (Basel) 2022-09, Vol.12 (9), p.2154
Main Authors: Lam, Anh Tung Phan, Sasaki, Kazunori, Yanagi, Yukiko, Tanaka, Shuhei, Ito, Shin-ichi
Format: Article
Language:English
Subjects:
Adaptability
Binding sites
Brassica
Cardamine occulta
clubroot disease
Crop diseases
Crops
Genes
Genetic diversity
Genetically altered foods
intronic distribution
Introns
Mutation
Open reading frames
Pathogenesis
Pathogens
Plasmodiophora brassicae
Polymorphism
Population
population diversity
Population genetics
Populations
Ribosomal DNA
rRNA
Splicing
Transversion
Weeds
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Summary:Analysis of the ribosomal introns of Plasmodiophora brassicae populations infecting the cruciferous weed Cardamine occulta revealed the complex dynamics of size, intraspecific variability, and distribution. The results showed that P. brassicae populations from the weed have lost multiple introns in the small and large subunits of the ribosomal RNA genes. Moreover, the retained introns, despite a largely mutual share of conserved parts with the cosmopolitan strains, contained numerous novel structures. These structural differences comprise a high level of polymorphisms, such as transversion point mutations occurring at sites involving the intronic splicing sites or insertions/deletions at the binding sites. Two geographical P. brassicae populations from C. occulta carried a lengthy intron-encoded ORF and putative mobile elements established in the large subunit. A few P. brassicae populations from the Brassica crops also harbored polymorphic introns that shared common mutated motifs with the weed-affecting group. The diversity of ribosomal introns observed from those investigated populations demonstrated the genetic distinction of the P. brassicae populations from C. occulta. The genetic variations might play a key role in the adaptability of the weed-infecting populations and are more likely related to the process of pathogenesis for the cosmopolitan P. brassicae infecting the Brassica crops.
ISSN:2073-4395
2073-4395
DOI:10.3390/agronomy12092154