Influence of Type of Substrate and Enzyme Concentration on Formation of Galacto-oligosaccharides

Different substrates and different concentrations of enzyme (Maxilact LX 5000) for galacto-oligosaccharides synthesis were tested. Lactose in phosphate buffer (138 mmol/l), ultrafiltration permeate (115 mmol/l), recombined whey (136 mmol/l) were used as substrates. Concentrations of used enzyme were...

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Bibliographic Details
Published in:Czech Journal of Food Sciences 2009-01, Vol.27 (Special Issue 1), p.S372-S374
Main Authors: Hellerová, K., Čurda, L.
Format: Article
Language:English
Subjects:
Buffers
Enzymes
galacto-oligosaccharides
Galactooligosaccharides
galactosidase
High-performance liquid chromatography
Lactose
Liquid chromatography
Oligosaccharides
permeate
Reaction products
Substrates
Ultrafiltration
Whey
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Summary:Different substrates and different concentrations of enzyme (Maxilact LX 5000) for galacto-oligosaccharides synthesis were tested. Lactose in phosphate buffer (138 mmol/l), ultrafiltration permeate (115 mmol/l), recombined whey (136 mmol/l) were used as substrates. Concentrations of used enzyme were from 0.15 to 15 U/ml for lactose in buffer, from 0.12 U/ml to 1.5 U/ml for ultrafiltration permeate and 1.5 U/ml for recombined whey. Reaction products were analysed by HPLC. There was obtained 6.4 ± 0.4 mmol/l of galacto-oligosaccharides (GOS) for lactose in buffer, it means that 0.0633 ± 0.0025 g/g of lactose was converted to GOS. The conversions of lactose to GOS for recombined whey and ultrafiltration permeate were 0.0669 ± 0.0079 and 0.0920 ± 0.0010 g/g. There was obtained 7.3 ± 0.1 mmol/l of GOS for ultrafiltration permeate and for recombined whey 5.9 ± 0.1 mmol/l of GOS.
ISSN:1212-1800
1805-9317
DOI:10.17221/960-CJFS