Porcine Breast Extracellular Matrix Hydrogel for Spatial Tissue Culture

Porcine mammary fatty tissues represent an abundant source of natural biomaterial for generation of breast-specific extracellular matrix (ECM). Here we report the extraction of total ECM proteins from pig breast fatty tissues, the fabrication of hydrogel and porous scaffolds from the extracted ECM p...

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Bibliographic Details
Published in:International journal of molecular sciences 2018-09, Vol.19 (10), p.2912
Main Authors: Rijal, Girdhari, Wang, Jing, Yu, Ilhan, Gang, David R, Chen, Roland K, Li, Weimin
Format: Article
Language:English
Subjects:
3D culture
Adipose Tissue - chemistry
Animals
Biocompatible Materials - chemistry
Breast - chemistry
Breast - cytology
Breast - metabolism
breast cancer
Cell Culture Techniques - methods
Cell Line
Cell Line, Tumor
Cell Movement
Cell Proliferation
Coculture Techniques - methods
Epithelial Cells - chemistry
Epithelial Cells - cytology
Epithelial Cells - metabolism
extracellular matrix
Extracellular Matrix Proteins - chemistry
Female
Humans
hydrogel
Hydrogels - chemistry
Metabolome
porcine
Porosity
scaffold
Swine
tissue matrix scaffold
Tissue Scaffolds - chemistry
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Summary:Porcine mammary fatty tissues represent an abundant source of natural biomaterial for generation of breast-specific extracellular matrix (ECM). Here we report the extraction of total ECM proteins from pig breast fatty tissues, the fabrication of hydrogel and porous scaffolds from the extracted ECM proteins, the structural properties of the scaffolds (tissue matrix scaffold, TMS), and the applications of the hydrogel in human mammary epithelial cell spatial cultures for cell surface receptor expression, metabolomics characterization, acini formation, proliferation, migration between different scaffolding compartments, and in vivo tumor formation. This model system provides an additional option for studying human breast diseases such as breast cancer.
ISSN:1422-0067
1422-0067
DOI:10.3390/ijms19102912